ArticleMolecular biotechnology1994
Enhancing PCR amplification and sequencing using DNA-binding proteins.
Article in Molecular biotechnology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.PloS one · 2022Article
- Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers.Scientific reports · 2018Article
- Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro.PloS one · 2018Article
- Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein.Scientific reports · 2017Article
- Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction.Scientific reports · 2016Article
- A Set of Assays for the Comprehensive Analysis of FMR1 Alleles in the Fragile X-Related Disorders.The Journal of molecular diagnostics : JMD · 2016Article
- DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication.Frontiers in molecular biosciences · 2016Review
- PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications.Scientific reports · 2014Article
- CdTe quantum dots enhance feasibility of EvaGreen-based real-time PCR with decent amplification fidelity.Molecular biotechnology · 2013Article
- Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning.Developmental biology · 2011Article
- Linear nicking endonuclease-mediated strand-displacement DNA amplification.Analytical biochemistry · 2011Article
- Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products.Nucleic acids research · 2005Article
- RNA amplification for successful gene profiling analysis.Journal of translational medicine · 2005Article
- Functional genomics and proteomics: application in neurosciences.Journal of neurology, neurosurgery, and psychiatry · 2004Review
- Signal and noise in bridging PCR.BMC biotechnology · 2002Article
- Quantitative analysis of mRNA amplification by in vitro transcription.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polymerase chain reaction (PCR) is a powerful core molecular biology technique, which when coupled to chain termination sequencing allows gene and DNA sequence information to be derived rapidly. A number of modifications to the basic PCR format have been developed in an attempt to increase amplification efficiency and the specificity of the reaction. We have applied the use of DNA-binding protein, gene 32 protein from bacteriophage T4 (T4gp32) to increase amplification efficiency with a number of diverse templates. In addition, we have found that using single-stranded DNA-binding protein (SSB) or recA protein in DNA sequencing reactions dramatically increases the resolution of sequencing runs. The use of DNA-binding proteins in amplification and sequencing may prove to be generally applicable in improving the yield and quality of a number of templates from various sources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.