ArticleThe Plant cell1994
Elevation of cytosolic calcium precedes anoxic gene expression in maize suspension-cultured cells.
Article in The Plant cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 181 citations in OpenAlex.
- Metabolic Profiling of Wheat Seedlings Under Oxygen Deficiency and Subsequent Reaeration Conditions.International journal of molecular sciences · 2025Article
- Hypoxia as challenge and opportunity: From cells to crops, to synthetic biology.Plant physiology · 2024Article
- Primed to persevere: Hypoxia regulation from epigenome to protein accumulation in plants.Plant physiology · 2024Review
- New Insights into the Connections between Flooding/Hypoxia Response and Plant Defenses against Pathogens.Plants (Basel, Switzerland) · 2024Review
- The Role of Aquaporins in Plant Growth under Conditions of Oxygen Deficiency.International journal of molecular sciences · 2022Review
- Potassium Efflux and Cytosol Acidification as Primary Anoxia-Induced Events in Wheat and Rice Seedlings.Plants (Basel, Switzerland) · 2020Article
- Exogenous Calcium Alleviates Nocturnal Chilling-Induced Feedback Inhibition of Photosynthesis by Improving Sink Demand in Peanut (Frontiers in plant science · 2020Article
- Mitochondrial Energy Signaling and Its Role in the Low-Oxygen Stress Response of Plants.Plant physiology · 2018Review
- Hydrogen sulfide enhances nitric oxide-induced tolerance of hypoxia in maize (Zea mays L.).Plant cell reports · 2016Article
- Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress.Plant physiology · 2016Article
- In vitro analyses of mitochondrial ATP/phosphate carriers from Arabidopsis thaliana revealed unexpected Ca(2+)-effects.BMC plant biology · 2015Article
- Reactive oxygen species-driven transcription in Arabidopsis under oxygen deprivation.Plant physiology · 2012Article
- Plant organellar calcium signalling: an emerging field.Journal of experimental botany · 2012Review
- Mechanisms for coping with submergence and waterlogging in rice.Rice (New York, N.Y.) · 2012Review
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- Coping with stresses: roles of calcium- and calcium/calmodulin-regulated gene expression.The Plant cell · 2011Review
- Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis.Journal of experimental botany · 2010Article
- The potato StLTPa7 gene displays a complex Ca-associated pattern of expression during the early stage of potato-Ralstonia solanacearum interaction.Molecular plant pathology · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Based on pharmacological evidence, we previously proposed that intracellular Ca2+ mediates the perception of O2 deprivation in maize seedlings. Herein, using fluorescence imaging and photometry of Ca2+ in maize suspension-cultured cells, the proposal was further investigated. Two complementary approaches were taken: (1) real time analysis of anoxia-induced changes in cytosolic Ca2+ concentration ([Ca]i) and (2) experimental manipulation of [Ca]i and then assay of the resultant anoxia-specific responses. O2 depletion caused an immediate increase in [Ca2+]i, and this was reversible within a few seconds of reoxygenation. The [Ca]i elevation proceeded independent of extracellular Ca2+. The kinetics of the Ca2+ response showed that it occurred much earlier than any detectable changes in gene expression. Ruthenium red blocked the anoxic [Ca]i elevation and also the induction of adh1 (encoding alcohol dehydrogenase) and sh1 (encoding sucrose synthase) mRNA. Ca2+, when added along with ruthenium red, prevented the effects of the antagonist on the anoxic responses. Verapamil and bepridil failed to block the [Ca]i rise induced by anoxia and were equally ineffective on anoxic gene expression. Caffeine induced an elevation of [Ca]i as well as ADH activity under normoxia. The data provide direct evidence for [Ca]i elevation in maize cells as a result of anoxia-induced mobilization of Ca2+ from intracellular stores. Furthermore, any manipulation that modified the [Ca]i rise brought about a parallel change in the expression of two anoxia-inducible genes. Thus, these results corroborate our proposal that [Ca]i is a physiological transducer of anoxia signals in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.