ArticleMolecular biology of the cell1994
The essential yeast Tcp1 protein affects actin and microtubules.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 107 citations in OpenAlex.
- TRiC/CCT chaperonin governs RNA polymerase II activity in the nucleus to support RNA homeostasis.Molecular cell · 2026Article
- Lethal effect of heterologous Haemonchus contortus α- and β-tubulin expression in Leishmania tarentolae.PloS one · 2026Article
- Nucleus Accumbens Proteome Disbalance in an Adolescent Mouse Model of Schizophrenia and Nicotine Misuse Comorbidity.Biomedicines · 2025Article
- Characterization of a protozoan Phosducin-like protein-3 (PhLP-3) reveals conserved redox activity.PloS one · 2018Article
- Genetic expansion of chaperonin-containing TCP-1 (CCT/TRiC) complex subunits yields testis-specific isoforms required for spermatogenesis in planarian flatworms.Molecular reproduction and development · 2017Article
- Defects in Protein Folding Machinery Affect Cell Wall Integrity and Reduce Ethanol Tolerance in S. cerevisiae.Current microbiology · 2016Article
- Cytoskeletal disruption activates the DLK/JNK pathway, which promotes axonal regeneration and mimics a preconditioning injury.Neurobiology of disease · 2015Article
- A pipeline for determining protein-protein interactions and proximities in the cellular milieu.Molecular & cellular proteomics : MCP · 2014Article
- Loss of the spectraplakin short stop activates the DLK injury response pathway in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Differential expression and redox proteomics analyses of an Alzheimer disease transgenic mouse model: effects of the amyloid-β peptide of amyloid precursor protein.Neuroscience · 2011Article
- An insertional trap for conditional gene expression in Toxoplasma gondii: identification of TAF250 as an essential gene.Molecular and biochemical parasitology · 2011Article
- Prefoldin 5 is required for normal sensory and neuronal development in a murine model.The Journal of biological chemistry · 2011Article
- Functional Subunits of Eukaryotic Chaperonin CCT/TRiC in Protein Folding.Journal of amino acids · 2011Article
- α-Tubulin mutations alter oryzalin affinity and microtubule assembly properties to confer dinitroaniline resistance.Eukaryotic cell · 2010Article
- Effect of TBCD and its regulatory interactor Arl2 on tubulin and microtubule integrity.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Article
- p90 ribosomal S6 kinase and p70 ribosomal S6 kinase link phosphorylation of the eukaryotic chaperonin containing TCP-1 to growth factor, insulin, and nutrient signaling.The Journal of biological chemistry · 2009Article
- Transcriptomic analysis of the exit from dormancy of Aspergillus fumigatus conidia.BMC genomics · 2008Article
- The interaction network of the chaperonin CCT.The EMBO journal · 2008Article
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Previously, we showed that the yeast Saccharomyces cerevisiae cold-sensitive mutation tcp1-1 confers growth arrest concomitant with cytoskeletal disorganization and disruption of microtubule-mediated processes. We have identified two new recessive mutations, tcp1-2 and tcp1-3, that confer heat- and cold-sensitive growth. Cells carrying tcp1 alleles were analyzed after exposure to the appropriate restrictive temperatures by cell viability tests, differential contrast microscopy, fluorescent, and immunofluorescent microscopy of DNA, tubulin, and actin and by determining the DNA content per cell. All three mutations conferred unique phenotypes indicative of cytoskeletal dysfunction. A causal relationship between loss of Tcp1p function and the development of cytoskeletal abnormalities was established by double mutant analyses. Novel phenotypes indicative of allele-specific genetic interactions were observed when tcp1-1 was combined in the same strain with tub1-1, tub2-402, act1-1, and act1-4, but not with other tubulin or actin mutations or with mutations in other genes affecting the cytoskeleton. Also, overproduction of wild-type Tcp1p partially suppressed growth defects conferred by act1-1 and act1-4. Furthermore, Tcp1p was localized to the cytoplasm and the cell cortex. Based on our results, we propose that Tcp1p is required for normal development and function of actin and microtubules either through direct or indirect interaction with the major cytoskeletal components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.