ArticlePlant molecular biology1995
Molecular cloning and characterization of profilin from tobacco (Nicotiana tabacum): increased profilin expression during pollen maturation.
Article in Plant molecular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 92 citations in OpenAlex.
- Profilin and Its Isoforms: Unravelling the Functional Diversity Across Life Forms.Current protein & peptide science · 2026Review
- Developmental and quantitative expression profile of the six pollen allergens of mugwort (Artemisia vulgaris L.).BMC plant biology · 2025Article
- Analysis of Pollen Allergens in Lily by Transcriptome and Proteome Data.International journal of molecular sciences · 2019Article
- Profilin as a regulator of the membrane-actin cytoskeleton interface in plant cells.Frontiers in plant science · 2013Review
- Article
- Characterization of PROFILIN genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors and expression analysis in cotton genotypes differing in fiber characteristics.Molecular biology reports · 2012Article
- Characterization of profilin polymorphism in pollen with a focus on multifunctionality.PloS one · 2012Article
- Profilin is essential for tip growth in the moss Physcomitrella patens.The Plant cell · 2007Article
- The function of actin-binding proteins in pollen tube growth.Protoplasma · 2007Review
- Signalling pathways in pollen germination and tube growth.Protoplasma · 2006Review
- Article
- Distinct roles of the first introns on the expression of Arabidopsis profilin gene family members.Plant physiology · 2006Article
- Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco.Plant molecular biology · 2005Article
- Biology of tree pollen allergens.Current allergy and asthma reports · 2004Review
- Identification of pronp1, a tobacco profilin gene activated in tip-growing cells.Plant molecular biology · 2001Article
- Profilin plays a role in cell elongation, cell shape maintenance, and flowering in Arabidopsis.Plant physiology · 2000Article
- Calcium-calmodulin suppresses the filamentous actin-binding activity of a 135-kilodalton actin-bundling protein isolated from lily pollen tubes.Plant physiology · 2000Article
- Signaling and the modulation of pollen tube growthThe Plant cell · 1999Article
- Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterizationPlant physiology · 1998Article
- Molecular cloning and mRNA localization of tomato pollen profilin.Plant molecular biology · 1998Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profilin has recently been identified as an actin-binding protein in higher plants. A cDNA coding for tobacco profilin, which shared an average sequence identity of 75% with other plant profilins, was isolated from a tobacco pollen cDNA library by antibody screening. Tobacco profilin was expressed in Escherichia coli and purified by affinity to poly-(L-proline) Sepharose. A rabbit antiserum was raised against recombinant tobacco profilin and used to estimate the amount of profilin expressed in different tobacco tissues. Profilin can be detected in different somatic tissues, but the expression is 50-100 fold higher in mature pollen. Immunofluorescence and confocal laser scanning microscopy showed a homogeneous distribution of profilin in the cytoplasm of in vitro cultured pollen grains and pollen tubes of tobacco whereas some growing pollen tubes were stained more intensively a their tip. A possible role of pollen profilin as a developmentally upregulated microfilament precursor in mature pollen is discussed.
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