ArticleProceedings of the National Academy of Sciences of the United States of America1995
Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 86 citations in OpenAlex.
- Conformational Alterations of the Cell Surface of Monomeric and Dimeric β2m-Free HLA-I (Proto-HLA) May Enable Novel Immune Functions in Health and Disease.Current issues in molecular biology · 2024Review
- Cell Surface B2m-Free Human Leukocyte Antigen (HLA) Monomers and Dimers: Are They Neo-HLA Class and Proto-HLA?Biomolecules · 2023Review
- Homotypic and heterotypicCurrent research in immunology · 2022Review
- MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells.Biophysical journal · 2016Article
- Dynamics of the actin cytoskeleton mediates receptor cross talk: An emerging concept in tuning receptor signaling.The Journal of cell biology · 2016Review
- B-cell receptor: from resting state to activate.Immunology · 2012Review
- Visualization of Protein Interactions in Living Cells.Self/nonself · 2011Article
- Palmitoylation of MIR2 is required for its function.Journal of virology · 2011Article
- AccPbFRET: an ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images.BMC bioinformatics · 2008Article
- Quantitative characterization of the large-scale association of ErbB1 and ErbB2 by flow cytometric homo-FRET measurements.Biophysical journal · 2008Article
- Visualization of protein interactions in living cells.Advances in experimental medicine and biology · 2008Review
- Influence of biologically inspired nanometer surface roughness on antigen-antibody interactions for immunoassay-biosensor applications.International journal of nanomedicine · 2006Article
- Article
- Dynamic, yet structured: The cell membrane three decades after the Singer-Nicolson model.Proceedings of the National Academy of Sciences of the United States of America · 2003Review
- Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Trafficking of spontaneously endocytosed MHC proteins.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Anomalous diffusion of major histocompatibility complex class I molecules on HeLa cells determined by single particle tracking.Biophysical journal · 1999Article
- Complexity of signal transduction mediated by ErbB2: clues to the potential of receptor-targeted cancer therapy.Pathology oncology research : POR · 1999Review
- Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer.The Journal of cell biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Major histocompatibility complex (MHC) class I antigens in the plasma membranes of human T (HUT-102B2) and B (JY) lymphoma cells were probed by immunochemical reagents using fluorescence, transmission electron, and scanning force microscopies. Fluorescent labels were attached to monoclonal antibodies W6/32 or KE-2 directed against the heavy chain of HLA class I (A, B, C) and L368 or HB28 against the beta 2-microglobulin light chain. The topological distribution in the nanometer range was studied by photobleaching fluorescence resonance energy transfer (pbFRET) on single cells. A nonrandom codistribution pattern of MHC class I molecules was observed over distances of 2-10 nm. A second, nonrandom, and larger-scale topological organization of the MHC class I antigens was detected by indirect immunogold labeling and imaging by transmission electron microscopy (TEM) and scanning force microscopy (SFM). Although some differences in antigen distribution between the B- and T-cell lines were detected by pbFRET, both cell lines exhibited similar clustering patterns by TEM and SFM. Such defined molecular distributions on the surfaces of cells of the immune system may reflect an underlying specialization of membrane lipid domains and fulfill important functional roles in cell-cell contacts and signal transduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.