ArticleMolecular and cellular biology1995
REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 37 citations in OpenAlex.
- Iron-mediated post-transcriptional regulation in Toxoplasma gondii.PLoS pathogens · 2025Article
- Review
- A CPF-like phosphatase module links transcription termination to chromatin silencing.Molecular cell · 2024Article
- A direct interaction between CPF and RNA Pol II links RNA 3' end processing to transcription.Molecular cell · 2023Article
- Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae.Nucleic acids research · 2008Article
- Article
- Identification of factors regulating poly(A) tail synthesis and maturation.Molecular and cellular biology · 2004Article
- The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3.Molecular and cellular biology · 2004Article
- In vivo evidence that defects in the transcriptional elongation factors RPB2, TFIIS, and SPT5 enhance upstream poly(A) site utilization.Molecular and cellular biology · 2003Article
- Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation.The EMBO journal · 2003Article
- The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae.Molecular biology of the cell · 2002Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- A mutation in GRS1, a glycyl-tRNA synthetase, affects 3'-end formation in Saccharomyces cerevisiae.Genetics · 1999Article
- RNA polymerase III defects suppress a conditional-lethal poly(A) polymerase mutation in Saccharomyces cerevisiae.Genetics · 1996Article
- RNA binding analysis of yeast REF2 and its two-hybrid interaction with a new gene product, FIR1.Gene expression · 1996Article
- Effects of mutations in the Saccharomyces cerevisiae RNA14, RNA15, and PAP1 genes on polyadenylation in vivo.Molecular and cellular biology · 1995Article
- The Paf1 complex: platform or player in RNA polymerase II transcription?Biochimica et biophysica actaReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae mutant ref2-1 (REF = RNA end formation) was originally identified by a genetic strategy predicted to detect decreases in the use of a CYC1 poly(A) site interposed within the intron of an ACT1-HIS4 fusion reporter gene. Direct RNA analysis now proves this effect and also demonstrates the trans action of the REF2 gene product on cryptic poly(A) sites located within the coding region of a plasmid-borne ACT1-lacZ gene. Despite impaired growth of ref2 strains, possibly because of a general defect in the efficiency of mRNA 3'-end processing, the steady-state characteristics of a variety of normal cellular mRNAs remain unaffected. Sequencing of the complementing gene predicts the Ref2p product to be a novel, basic protein of 429 amino acids (M(r), 48,000) with a high-level lysine/serine content and some unusual features. Analysis in vitro, with a number of defined RNA substrates, confirms that efficient use of weak poly(A) sites requires Ref2p: endonucleolytic cleavage is carried out accurately but at significantly lower rates in extracts prepared from delta ref2 cells. The addition of purified, epitope-tagged Ref2p (Ref2pF) reestablishes wild-type levels of activity in these extracts, demonstrating direct involvement of this protein in the cleavage step of 3' mRNA processing. Together with the RNA-binding characteristics of Ref2pF in vitro, our results support an important contributing role for the REF2 locus in 3'-end processing. As the first gene genetically identified to participate in mRNA 3'-end maturation prior to the final polyadenylation step, REF2 provides an ideal starting point for identifying related genes in this event.
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