ArticleMolecular and cellular biology1995
Analysis of the yeast transcription factor TFIIA: distinct functional regions and a polymerase II-specific role in basal and activated transcription.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 95 citations in OpenAlex.
- Mechanisms and Functions of the RNA Polymerase II General Transcription Machinery during the Transcription Cycle.Biomolecules · 2024Review
- Network pharmacology-based research on the effect of Radix Astragali on osteosarcoma and the underlying mechanism.Scientific reports · 2023Article
- Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1.Current genetics · 2023Article
- Comparison of growth performance and related gene expression of muscle and fat from Landrace, Yorkshire, and Duroc and Woori black pigs.Journal of animal science and technology · 2023Article
- DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability.Nature communications · 2020Article
- TASP1 mutation in a female with craniofacial anomalies, anterior segment dysgenesis, congenital immunodeficiency and macrocytic anemia.Molecular genetics & genomic medicine · 2019Article
- Structural basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2015Review
- Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.Transcription · 2015Article
- Direct TFIIA-TFIID protein contacts drive budding yeast ribosomal protein gene transcription.The Journal of biological chemistry · 2013Article
- Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.Nucleic acids research · 2011Article
- Molecular characterization of ScTFIIAgamma, encoding the putative TFIIA small subunit from sugarcane.Plant cell reports · 2010Article
- Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II.Nucleic acids research · 2008Article
- TFIIA plays a role in the response to oxidative stress.Eukaryotic cell · 2006Article
- Characterization of a multisubunit transcription factor complex essential for spliced-leader RNA gene transcription in Trypanosoma brucei.Molecular and cellular biology · 2005Article
- Mapping and functional characterization of the TAF11 interaction with TFIIA.Molecular and cellular biology · 2005Article
- Ectopic expression of URA3 can influence the virulence phenotypes and proteome of Candida albicans but can be overcome by targeted reintegration of URA3 at the RPS10 locus.Eukaryotic cell · 2004Article
- Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA.Genes & development · 2004Article
- Multi-protein complexes in eukaryotic gene transcription.Plant molecular biology · 2002Review
- Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- TAF(II)170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
To probe the structure and function of the Saccharomyces cerevisiae general transcription factor TFIIA, we have systematically mutagenized the genes encoding both subunits and analyzed the effects of the mutations both in vivo and in vitro. We found that the central nonconserved region of the large subunit is not essential for function and likely acts as a spacer between the conserved N- and C-terminal regions. Deletion mutagenesis of the large subunit defined a region which is required for TATA binding protein (TBP) interaction. Alanine scanning mutagenesis defined a cluster of four basic residues which are likely required for interaction with DNA in the TBP-DNA complex. Much of the conserved regions of both subunits is required for subunit association, suggesting that these conserved regions fold into compact domains which extensively interact. In vitro transcription performed with extracts from yeast strains with mutations in either the large or the small TFIIA subunit demonstrated that TFIIA stimulates both basal and activated polymerase II (Pol II) transcription. The TFIIA-depleted extracts have normal Pol I and Pol III transcription activity, showing that TFIIA is a Pol II-specific factor. In vivo depletion of TFIIA activity reduced transcription from four different Pol II promoters. Finally, alanine scanning mutagenesis of TFIIA's small subunit has identified at least one mutation which is defective in transcription but which is not defective in subunit association or binding to TBP or TBP-DNA complexes.
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