ArticleMolecular and cellular biology1995
Autoregulation of the human C/EBP alpha gene by stimulation of upstream stimulatory factor binding.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 152 citations in OpenAlex.
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- ZNF143 protein is an important regulator of the myeloid transcription factor C/EBPα.The Journal of biological chemistry · 2017Article
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- Immunological evidence and regulatory potential for cell-penetrating antibodies in intravenous immunoglobulin.Clinical & translational immunology · 2015Article
- Frequency and prognostic impact of CEBPA proximal, distal and core promoter methylation in normal karyotype AML: a study on 623 cases.PloS one · 2013Article
- Overexpression of TRIB2 in human lung cancers contributes to tumorigenesis through downregulation of C/EBPα.Oncogene · 2011Article
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- Computational modeling of the hematopoietic erythroid-myeloid switch reveals insights into cooperativity, priming, and irreversibility.PLoS computational biology · 2009Article
- Proteomic discovery of Max as a novel interacting partner of C/EBPalpha: a Myc/Max/Mad link.Leukemia · 2006Article
- TFII-I regulates induction of chromosomally integrated human immunodeficiency virus type 1 long terminal repeat in cooperation with USF.Journal of virology · 2005Article
- Potential autoregulation of transcription factor PU.1 by an upstream regulatory element.Molecular and cellular biology · 2005Article
- GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells.Nucleic acids research · 2005Article
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- Regulatory potential scores from genome-wide three-way alignments of human, mouse, and rat.Genome research · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The human C/EBP alpha gene promoter shares significant sequence homology with that of the mouse but has a different mechanism of autoregulation. Activation of the murine promoter by direct binding of C/EBP alpha to a site within 200 bp of the transcriptional start was shown to elevate activity by approximately threefold (R. J. Christy, K. H. Kaestner, D. E. Geiman, and M. D. Lane, Proc. Natl. Acad. Sci. USA 88:2593-2597, 1991; K. Legraverend, P. Antonson, P. Flodby, and K. G. Xanthapoulos, Nucleic Acids Res. 21:1735-1742, 1993). Unlike its murine counterpart, the human C/EBP alpha gene promoter does not contain a cis element that binds the C/EBP alpha protein. Neither C/EBP alpha nor C/EBP beta (NF-Il-6) binds the human C/EBP alpha promoter within 437 bp. However, cotransfection studies show that C/EBP alpha stimulates transcription of a reporter gene driven by 437 bp of the C/EBP alpha promoter. Our studies show that the human C/EBP alpha protein stimulates USF to bind to a USF consensus element within C/EBP alpha promoter and activates it by two- to threefold. We propose that the human gene employs the ubiquitously expressed DNA-binding protein factor USF to carry out autoregulation. Autoregulation of the human C/EBP alpha promoter was abolished by deletion of the USF binding site, CACGTG. Expression of human C/EBP beta following transfection did not stimulate USF binding. These studies suggest a mechanism whereby tissue-specific autoregulation can be achieved via a trans-acting factor that is expressed in all cell types. Thus, direct binding of the C/EBP alpha protein to the promoter of the C/EBP alpha gene is not required for autoregulation.
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