ArticleThe Journal of cell biology1995
Regulation of cortical actin cytoskeleton assembly during polarized cell growth in budding yeast.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 156 citations in OpenAlex.
- Please inhibit responsibly: Natural and synthetic actin toxins as useful tools in cell biology.Molecular biology of the cell · 2025Review
- Genomic signals found using RNA sequencing show signatures of selection and subtle population differentiation in walleye (Ecology and evolution · 2020Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Rong Li: tipping the balance in cell biology. Interview by Caitlin Sedwick.The Journal of cell biology · 2011Article
- Characterization of PCEng2, a {beta}-1,3-endoglucanase homolog in Pneumocystis carinii with activity in cell wall regulation.American journal of respiratory cell and molecular biology · 2010Article
- Dual modes of cdc42 recycling fine-tune polarized morphogenesis.Developmental cell · 2009Article
- SLA2 mutations cause SWE1-mediated cell cycle phenotypes in Candida albicans and Saccharomyces cerevisiae.Microbiology (Reading, England) · 2009Article
- Review of the mechanism of processive actin filament elongation by formins.Cell motility and the cytoskeleton · 2009Review
- Central roles of small GTPases in the development of cell polarity in yeast and beyond.Microbiology and molecular biology reviews : MMBR · 2007Review
- Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast.Molecular biology of the cell · 2005Article
- Yeast actin patches are networks of branched actin filaments.The Journal of cell biology · 2004Article
- Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.The Journal of cell biology · 2004Article
- Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast.Molecular biology of the cell · 2003Article
- The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe.The Journal of cell biology · 2003Article
- Mum, this bud's for you: where do you want it? Roles for Cdc42 in controlling bud site selection in Saccharomyces cerevisiae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2003Review
- Rapid de-localization of actin leading edge components with BDM treatment.BMC cell biology · 2003Article
- Synthetic lethal analysis implicates Ste20p, a p21-activated potein kinase, in polarisome activation.Molecular biology of the cell · 2003Article
- Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- A role for cell polarity proteins in mitotic exit.The EMBO journal · 2002Article
- Cdc42p functions at the docking stage of yeast vacuole membrane fusion.The EMBO journal · 2001Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have established an in vitro assay for assembly of the cortical actin cytoskeleton of budding yeast cells. After permeabilization of yeast by a novel procedure designed to maintain the spatial organization of cellular constituents, exogenously added fluorescently labeled actin monomers assemble into distinct structures in a pattern that is similar to the cortical actin distribution in vivo. Actin assembly in the bud of small-budded cells requires a nucleation activity provided by protein factors that appear to be distinct from the barbed ends of endogenous actin filaments. This nucleation activity is lost in cells that lack either Sla1 or Sla2, proteins previously implicated in cortical actin cytoskeleton function, suggesting a possible role for these proteins in the nucleation reaction. The rate and the extent of actin assembly in the bud are increased in permeabilized delta cap2 cells, providing evidence that capping protein regulates the ability of the barbed ends of actin filaments to grow in yeast cells. Actin incorporation in the bud can be stimulated by treating the permeabilized cells with GTP-gamma S, and, significantly, the stimulatory effect is eliminated by a mutation in CDC42, a gene that encodes a Rho-like GTP-binding protein required for bud formation. Furthermore, the lack of actin nucleation activity in the cdc42 mutant can be complemented in vitro by a constitutively active Cdc42 protein. These results suggest that Cdc42 is closely involved in regulating actin assembly during polarized cell growth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.