ArticleThe EMBO journal1995
Random activation of a transgene under the control of a hybrid hCD2 locus control region/Ig enhancer regulatory element.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.Molecular cell · 2017Article
- The use of mouse models to study epigenetics.Cold Spring Harbor perspectives in biology · 2013Review
- A 3,387 bp 5'-flanking sequence of the goat alpha-S1-casein gene provides correct tissue-specific expression of human granulocyte colony-stimulating factor (hG-CSF) in the mammary gland of transgenic mice.Transgenic research · 2012Article
- Mono-allelic expression of variegating transgene locus in the mouse.Transgenic research · 2003Article
- Increased noise as an effect of haploinsufficiency of the tumor-suppressor gene neurofibromatosis type 1 in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration.The EMBO journal · 2002Article
- In vivo analysis of retroviral enhancer mutations in hematopoietic cells: SP1/EGR1 and ETS/GATA motifs contribute to long terminal repeat specificity.Journal of virology · 2002Article
- Article
- Adjacent DNA elements dominantly restrict the ubiquitous activity of a novel chromatin-opening region to specific tissues.The EMBO journal · 1997Article
- Natural killer cell tolerance in mice with mosaic expression of major histocompatibility complex class I transgene.The Journal of experimental medicine · 1997Article
- Chromatin domains and nuclear compartments: establishing sites of gene expression in eukaryotic nuclei.Molecular biology reports · 1997Review
- Recent advances in transgenic technology.Molecular biotechnology · 1997Review
- A globin enhancer acts by increasing the proportion of erythrocytes expressing a linked transgene.Molecular and cellular biology · 1997Article
- A transgenic marker for mouse B lymphoid precursors.The Journal of experimental medicine · 1997Article
- Stochastic mechanisms in gene expression.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Do transgene arrays form heterochromatin in vertebrates?Transgenic research · 1997Review
- Sheltering of gamma-globin expression from position effects requires both an upstream locus control region and a regulatory element 3' to the A gamma-globin gene.Molecular and cellular biology · 1997Article
- Variegated expression of a globin transgene correlates with chromatin accessibility but not methylation status.Nucleic acids research · 1996Article
- Age-dependent silencing of globin transgenes in the mouse.Nucleic acids research · 1996Article
- Genomic position effects lead to an inefficient reorganization of nucleosomes in the 5'-regulatory region of the chicken lysozyme locus in transgenic mice.Nucleic acids research · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Locus control regions such as those of human CD2 and beta-globin differ from classical enhancers in that, whereas the former confer high level, copy-dependent, position-independent expression to linked genes in transgenic mice, the latter do not, expression levels being dependent on the site of integration. We report that the position independence of the CD2 locus control region is modified by coupling it to the immunoglobulin heavy chain enhancer. Whilst in the majority of transgenic lines the Ig heavy chain enhancer has little or no effect on T cell expression of the hCD2 transgene, in others transgene expression is non-specifically extinguished in a proportion of lymphoid cells. The transgenic locus chromatin appears inaccessible to DNase I in these cells, which do not express the gene. Furthermore, mice homozygous for the hybrid hCD2-Ig heavy chain enhancer construct contain T cells with both an active and an inactive transgene. The 'decision' to express or repress the gene appears to be a random process which involves each chromosome separately, occurs at early stages in differentiation and is heritable by daughter cells. These data suggest the possibility that stochastic decisions might control a number of biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.