ArticleThe EMBO journal1995
Suppression of villin expression by antisense RNA impairs brush border assembly in polarized epithelial intestinal cells.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 127 citations in OpenAlex.
- AnFrontiers in immunology · 2024Article
- Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells.PLoS neglected tropical diseases · 2017Article
- Directional Fluid Transport across Organ-Blood Barriers: Physiology and Cell Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Plasticity of the brush border - the yin and yang of intestinal homeostasis.Nature reviews. Gastroenterology & hepatology · 2016Review
- Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells.Beilstein journal of nanotechnology · 2015Article
- Article
- Medaka villin 1-like protein (VILL) is associated with the formation of microvilli induced by decreasing salinities in the absorptive ionocytes.Frontiers in zoology · 2014Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Extracellular nucleotides inhibit oxalate transport by human intestinal Caco-2-BBe cells through PKC-δ activation.American journal of physiology. Cell physiology · 2013Article
- Antisense RNA regulation and application in the development of novel antibiotics to combat multidrug resistant bacteria.Science progress · 2013Review
- Intestinal mucosal atrophy and adaptation.World journal of gastroenterology · 2012Review
- Villin expression is frequently lost in poorly differentiated colon cancer.The American journal of pathology · 2012Article
- A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.Molecular biology of the cell · 2012Article
- Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.Cytotechnology · 2011Article
- Regulation of villin by wnt5a/ror2 signaling in human intestinal cells.Frontiers in physiology · 2011Article
- Loss of the actin remodeler Eps8 causes intestinal defects and improved metabolic status in mice.PloS one · 2010Article
- Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium.Molecular biology of the cell · 2009Article
- Dynamical control of the shape and size of stereocilia and microvilli.Biophysical journal · 2007Article
- Villin severing activity enhances actin-based motility in vivo.Molecular biology of the cell · 2007Article
- Antigen transport and cytoskeletal characteristics of a distinct enterocyte population in inflammatory bowel diseases.The American journal of pathology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used an antisense RNA strategy to investigate the role of the actin-associated protein, villin, in the brush-border morphogenesis of human intestinal CaCO2 cells. Stable expression of a cDNA encoding antisense villin RNA resulted in the permanent down-regulation of the endogenous villin message and dramatically affected brush-border assembly. Ultrastructural and immunolocalization studies revealed that epithelial cell polarity was largely maintained. However, in contrast to brush-border markers such as dipeptidyl-peptidase IV, the apical localization of sucrase-isomaltase was specifically impaired. Retransfection of the villin antisense-expressing cell line with a cDNA encoding a partial sense villin RNA restored both brush-border assembly and sucrase-isomaltase apical expression. The suggestion that brush-border morphogenesis may be important for the trafficking of certain proteins is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.