ArticlePlant molecular biology1994
Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco.
Article in Plant molecular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 134 citations in OpenAlex.
- Gpa2 detects the potato cyst nematode effector GpRBP-1 in the cytoplasm but requires a balanced nucleocytoplasmic distribution to trigger cell death.Journal of experimental botany · 2025Article
- Sequence Exchange between Homologous NB-LRR Genes Converts Virus Resistance into Nematode Resistance, and Vice Versa.Plant physiology · 2017Article
- Transient Expression of Secretory IgA In Planta is Optimal Using a Multi-Gene Vector and may be Further Enhanced by Improving Joining Chain Incorporation.Frontiers in plant science · 2015Article
- Identification, Characterization and Down-Regulation of Cysteine Protease Genes in Tobacco for Use in Recombinant Protein Production.PloS one · 2015Article
- Attainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display.Molecular biotechnology · 2012Article
- The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants.Plant physiology · 2012Article
- 3D domain swapping causes extensive multimerisation of human interleukin-10 when expressed in planta.PloS one · 2012Article
- Antibody degradation in tobacco plants: a predominantly apoplastic process.BMC biotechnology · 2011Article
- Evolution of plant-made pharmaceuticals.International journal of molecular sciences · 2011Review
- Nucleocytoplasmic distribution is required for activation of resistance by the potato NB-LRR receptor Rx1 and is balanced by its functional domains.The Plant cell · 2010Article
- Different subcellular localization and glycosylation for a functional antibody expressed in Nicotiana tabacum plants and suspension cells.Transgenic research · 2009Article
- Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants.Plant molecular biology · 2003Article
- Assembly of two transgenes in an artificial chromatin domain gives highly coordinated expression in tobacco.Genetics · 2002Article
- Enabling technologies for manipulating multiple genes on complex pathways.Plant molecular biology · 2001Review
- Influence of growth conditions and developmental stage on N-glycan heterogeneity of transgenic immunoglobulin G and endogenous proteins in tobacco leaves.Plant physiology · 2001Article
- Antibody-based resistance to plant pathogens.Transgenic research · 2001Review
- Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco.Plant physiology · 2000Article
- Plants as bioreactors for protein production: avoiding the problem of transgene silencing.Plant molecular biology · 2000Review
- Molecular farming of pharmaceutical proteins.Transgenic research · 2000Review
- Use of plant roots for phytoremediation and molecular farming.Proceedings of the National Academy of Sciences of the United States of America · 1999Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To explore the feasibility of employing antibodies to obtain disease resistance against plant root pathogens, we have studied the expression of genes encoding antibodies in roots of transgenic plants. A model monoclonal antibody was used that binds to a fungal cutinase. Heavy and light chain cDNAs were amplified by PCR, fused to a signal sequence for secretion and cloned behind CaMV 35S and TR2' promoters in a single T-DNA. The chimeric genes were cloned both in tandem and in a divergent orientation. The roots of tobacco plants transformed with these constructs produced antibodies that were able to bind antigen in an ELISA. Immunoblotting showed assembly to a full-size antibody. In addition, a F(ab')2-like fragment was observed, which is probably formed by proteolytic processing. Both antibody species were properly targeted to the apoplast, but the full-size antibody was partially retained by the wall of suspension cells. The construct with divergent promoters showed a better performance than the construct with promoters in tandem. It directed the accumulation of functional antibodies to a maximum of 1.1% of total soluble protein, with half of the plants having levels higher than 0.35%. The high efficiency of this construct probably results from coordinated and balanced expression of light and heavy chain genes, as evidenced by RNA blot hybridization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.