ReviewPlant molecular biology1994
Activation tagging: a means of isolating genes implicated as playing a role in plant growth and development.
Review in Plant molecular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 112 citations in OpenAlex.
- Misexpression Approaches for the Manipulation of Flower Development.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Gain-of-function mutations: key tools for modifying or designing novel proteins in plant molecular engineering.Journal of experimental botany · 2020Article
- Exploring Genetic Diversity in Plants Using High-Throughput Sequencing Techniques.Current genomics · 2016Article
- Gene Overexpression Resources in Cereals for Functional Genomics and Discovery of Useful Genes.Frontiers in plant science · 2016Review
- Characterization of a potato activation-tagged mutant, nikku, and its partial revertant.Planta · 2015Article
- Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.PloS one · 2014Article
- An active ac/ds transposon system for activation tagging in tomato cultivar m82 using clonal propagation.Plant physiology · 2013Article
- Transposon activation mutagenesis as a screening tool for identifying resistance to cancer therapeutics.BMC cancer · 2013Article
- Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice.Plant molecular biology · 2007Article
- Hairy root-activation tagging: a high-throughput system for activation tagging in transformed hairy roots.Plant molecular biology · 2005Article
- Reverse genetic approaches for functional genomics of rice.Plant molecular biology · 2005Review
- Structural and functional analysis of rice genome.Journal of genetics · 2004Review
- Cytoplasmic N-terminal protein acetylation is required for efficient photosynthesis in Arabidopsis.The Plant cell · 2003Article
- Activation tagging using the En-I maize transposon system in Arabidopsis.Plant physiology · 2002Article
- Finding your knockout: reverse genetics techniques for plants.Molecular biotechnology · 2002Article
- Arabidopsis genes essential for seedling viability: isolation of insertional mutants and molecular cloning.Genetics · 2001Article
- BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A plant cation-chloride co-transporter promoting auxin-independent tobacco protoplast division.The EMBO journal · 1997Article
- High level transcription of a member of a repeated gene family confers dehydration tolerance to callus tissue of Craterostigma plantagineum.The EMBO journal · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation T-DNA tagging has been used to generate a variety of tobacco cell lines selected by their ability to grow either in the absence of auxin or cytokinin in the culture media, or under selective levels of an inhibitor of polyamine biosynthesis. The majority of the cell lines studied in detail contain single T-DNA inserts genetically co-segregating with the selected phenotype. While most of the plants regenerated from the mutant cell lines appear phenotypically normal, several display phenotypes which could be inferred to result from disturbances in the content, or the metabolism, of auxins and cytokinins, or polyamines. The tagging vector is designed to allow the isolation of tagged plant genes by plasmid rescue. Confirmation that the genomic sequence responsible for the selected phenotype has indeed been isolated is provided by PEG-mediated protoplast DNA uptake of rescued plasmids followed by selection for protoplast growth under the original selective conditions. Several plasmids have been rescued from the mutant lines which confer on transfected protoplasts the ability to grow either in the absence of auxin or cytokinin in the culture media, or under selective levels of an inhibitor of polyamine biosynthesis. This review describes the background to activation tagging and our progress in characterizing the genes that have been tagged in the mutant lines we have generated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.