ArticleBiophysical journal1994
Formation of two-dimensional complexes of F-actin and crosslinking proteins on lipid monolayers: demonstration of unipolar alpha-actinin-F-actin crosslinking.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 77 citations in OpenAlex.
- SimHS-AFMfit-MD: An Integrative Approach for Inferring Alpha-Actinin Atomic Conformational Dynamics.Nano letters · 2026Article
- A two-way street - cellular metabolism and myofibroblast contraction.NPJ Regenerative medicine · 2024Review
- Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization.Scientific reports · 2022Article
- Crosstalk between mechanotransduction and metabolism.Nature reviews. Molecular cell biology · 2021Review
- Spatial high resolution of actin filament organization by PeakForce atomic force microscopy.Cell proliferation · 2020Article
- Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration.Scientific reports · 2019Article
- Adducins inhibit lung cancer cell migration through mechanisms involving regulation of cell-matrix adhesion and cadherin-11 expression.Biochimica et biophysica acta. Molecular cell research · 2019Article
- Non-canonical functions of enzymes facilitate cross-talk between cell metabolic and regulatory pathways.Experimental & molecular medicine · 2018Review
- Identification of interfaces involved in weak interactions with application to F-actin-aldolase rafts.Journal of structural biology · 2018Article
- A Review on Adducin from Functional to Pathological Mechanisms: Future Direction in Cancer.BioMed research international · 2018Review
- Human myosin VIIa is a very slow processive motor protein on various cellular actin structures.The Journal of biological chemistry · 2017Article
- Adducin is required for desmosomal cohesion in keratinocytes.The Journal of biological chemistry · 2014Article
- Filament-filament switching can be regulated by separation between filaments together with cargo motor number.PloS one · 2013Article
- Article
- Spatial localisation of actin filaments across developmental stages of the malaria parasite.PloS one · 2012Article
- Spectrin-adducin membrane skeleton: A missing link between epithelial junctions and the actin cytoskeletion?Bioarchitecture · 2011Article
- Adducins regulate remodeling of apical junctions in human epithelial cells.Molecular biology of the cell · 2010Article
- Article
- The 3D structure of villin as an unusual F-Actin crosslinker.Structure (London, England : 1993) · 2008Article
- Novel structures for alpha-actinin:F-actin interactions and their implications for actin-membrane attachment and tension sensing in the cytoskeleton.Journal of molecular biology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A method is described for forming two-dimensional (2-D) paracrystalline complexes of F-actin and bundling/gelation proteins on positively charged lipid monolayers. These arrays facilitate detailed structural studies of protein interactions with F-actin by eliminating superposition effects present in 3-D bundles. Bundles of F-actin have been produced using the glycolytic enzymes aldolase and glyceraldehyde-3-phosphate dehydrogenase, the cytoskeletal protein erythrocyte adducin as well as smooth muscle alpha-actinin from chicken gizzard. All of the 2-D bundles formed contain F-actin with a 13/6 helical structure. F-actin-aldolase bundles have an interfilament spacing of 12.6 nm and a superlattice arrangement of actin filaments that can be explained by expression of a local twofold axis in the neighborhood of the aldolase. Well ordered F-actin-alpha-actinin 2-D bundles have an interfilament spacing of 36 nm and contain crosslinks 33 nm in length angled approximately 25-35 degrees to the filament axis. Images and optical diffraction patterns of these bundles suggest that they consist of parallel, unipolar arrays of actin filaments. This observation is consistent with an actin crosslinking function at adhesion plaques where actin filaments are bound to the cell membrane with uniform polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.