ArticleBiophysical journal1994
Structural connectivity in actin: effect of C-terminal modifications on the properties of actin.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 94 citations in OpenAlex.
- Actin's functional "switch": Constraining C-terminal conformational flexibility disrupts functionally important communication networks.The Journal of biological chemistry · 2026Article
- A structural model of the profilin-formin pacemaker system for actin filament elongation.Scientific reports · 2022Article
- Photoredox-Catalyzed DecarboxylativeACS chemical biology · 2021Article
- Myopathy-Sensitive G-Actin Segment 227-235 Is Involved in Salt-Induced Stabilization of Contacts within the Actin Filament.International journal of molecular sciences · 2021Article
- Review
- High-throughput screen, using time-resolved FRET, yields actin-binding compounds that modulate actin-myosin structure and function.The Journal of biological chemistry · 2018Article
- Plasmodium actin is incompletely folded by heterologous protein-folding machinery and likely requires the native Plasmodium chaperonin complex to enter a mature functional state.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Structure of the F-actin-tropomyosin complex.Nature · 2015Article
- Near-atomic resolution for one state of F-actin.Structure (London, England : 1993) · 2015Article
- Functional analysis of a de novo ACTB mutation in a patient with atypical Baraitser-Winter syndrome.Human mutation · 2013Article
- Structural states and dynamics of the D-loop in actin.Biophysical journal · 2012Article
- Regulation of cell physiology and pathology by protein S-glutathionylation: lessons learned from the cardiovascular system.Antioxidants & redox signaling · 2012Review
- Myosin binding surface on actin probed by hydroxyl radical footprinting and site-directed labels.Journal of molecular biology · 2011Article
- Redox signaling and cardiac sarcomeres.The Journal of biological chemistry · 2011Review
- A nucleotide state-sensing region on actin.The Journal of biological chemistry · 2010Article
- Control of the ability of profilin to bind and facilitate nucleotide exchange from G-actin.The Journal of biological chemistry · 2008Article
- Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers.American journal of physiology. Cell physiology · 2008Article
- Role of actin DNase-I-binding loop in myosin subfragment 1-induced polymerization of G-actin: implications for the mechanism of polymerization.Biophysical journal · 2005Article
- Cofilin and DNase I affect the conformation of the small domain of actin.Biophysical journal · 2002Article
- Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor.Biophysical journal · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In this study, we use fluorescent probes and proteolytic digestions to demonstrate structural coupling between distant regions of actin. We show that modifications of Cys-374 in the C-terminus of actin slow the rate of nucleotide exchange in the nucleotide cleft. Conformational coupling between the C-terminus and the DNasal loop in subdomain II is observed in proteolytic digestion experiments in which a new C-terminal cleavage site is exposed upon DNasel binding. The functional consequences of C-terminal modification are evident from S-1 ATPase activity and the in vitro motility experiments with modified actins. Pyrene actin, labeled at Cys-374, activates S-1 ATPase activity only half as well as control actin. This reduction is attributed to a lower Vmax value because the affinity of pyrene actin to S-1 is not significantly altered. The in vitro sliding velocity of pyrene actin is also decreased. However, IAEDANS labeling of actin (also at Cys-374) enhances the Vmax of acto-S-1 ATPase activity and the in vitro sliding velocity by approximately 25%. These results are discussed in terms of conformational coupling between distant regions in actin and the functional implications of the interactions of actin-binding proteins with the C-terminus of actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.