ArticleJournal of virology1995
Purification and characterization of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Nucleocytoplasmic Shuttling of Porcine Parvovirus NS1 Protein Mediated by the CRM1 Nuclear Export Pathway and the Importin α/β Nuclear Import Pathway.Journal of virology · 2022Article
- Canine Parvovirus and Its Non-Structural Gene 1 as Oncolytic Agents: Mechanism of Action and Induction of Anti-Tumor Immune Response.Frontiers in oncology · 2021Review
- The 5' Untranslated Region of the Capsid Protein 2 Gene of Mink Enteritis Virus Is Essential for Its Expression.Journal of virology · 2018Article
- Genetic characterization of the complete genome of an Aleutian mink disease virus isolated in north China.Virus genes · 2016Article
- Amdoparvoviruses in small mammals: expanding our understanding of parvovirus diversity, distribution, and pathology.Frontiers in microbiology · 2015Review
- Molecular epidemiology of Aleutian disease virus in free-ranging domestic, hybrid, and wild mink.Evolutionary applications · 2012Article
- Genomic features of the human bocaviruses.Future virology · 2012Article
- Article
- Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein.Journal of virology · 2006Article
- The recombinant nonstructural polyprotein NS1 of porcine parvovirus (PPV) as diagnostic antigen in ELISA to differentiate infected from vaccinated pigs.Veterinary research communications · 2006Article
- Novel PKCeta is required to activate replicative functions of the major nonstructural protein NS1 of minute virus of mice.Journal of virology · 2003Article
- Caspase cleavage of the nonstructural protein NS1 mediates replication of Aleutian mink disease parvovirus.Journal of virology · 2003Article
- Human parvovirus B19.Clinical microbiology reviews · 2002Review
- Minute virus of mice initiator protein NS1 and a host KDWK family transcription factor must form a precise ternary complex with origin DNA for nicking to occur.Journal of virology · 2001Article
- DNA unwinding functions of minute virus of mice NS1 protein are modulated specifically by the lambda isoform of protein kinase C.Journal of virology · 1999Article
- Replicative functions of minute virus of mice NS1 protein are regulated in vitro by phosphorylation through protein kinase C.Journal of virology · 1998Article
- Biochemical activities of minute virus of mice nonstructural protein NS1 are modulated In vitro by the phosphorylation state of the polypeptide.Journal of virology · 1998Article
- A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication.Journal of virology · 1997Article
- Specific initiation of replication at the right-end telomere of the closed species of minute virus of mice replicative-form DNA.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously described the expression of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus (ADV) in insect cells by using a baculovirus vector (J. Christensen, T. Storgaard, B. Bloch, S. Alexandersen, and B. Aasted, J. Virol. 67:229-238, 1993). To study its biochemical properties, ADV NS-1 was expressed in Sf9 insect cells and purified to apparent homogeneity with a combination of nuclear extraction, Zn2+ ion chromatography, and immunoaffinity chromatography on monoclonal antibodies. The purified protein showed ATP binding and ATPase- and ATP- or dATP-dependent helicase activity requiring either Mg2+ or Mn2+ as a cofactor. The ATPase activity of NS-1 was efficiently stimulated by single-stranded DNA and, to a lesser extent, double-stranded DNA. We also describe the expression, purification, and characterization of a mutant NS-1 protein, in which a lysine in the putative nucleotide binding consensus sequence of the molecule was replaced with serine. The mutated NS-1 was expressed at 10-fold higher levels than wild-type NS-1, but it exhibited no ATP binding. ATPase, or helicase activity. The availability of large amounts of purified functional NS-1 protein will facilitate studies of the biochemistry of ADV replication and gene regulation leading to disease in mink.
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