ArticleJournal of virology1995
Analysis and mapping of a family of 3'-coterminal transcripts containing coding sequences for human cytomegalovirus open reading frames UL93 through UL99.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- Review
- Human Cytomegalovirus Protein UL94 Targets MITA to Evade the Antiviral Immune Response.Journal of virology · 2020Article
- Human Cytomegalovirus Primary Infection and Reactivation: Insights From Virion-Carried Molecules.Frontiers in microbiology · 2020Review
- Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.Journal of virology · 2016Article
- A cluster of 3' coterminal transcripts from US12-US17 locus of human cytomegalovirus.Virus genes · 2016Article
- Identification of human cytomegalovirus genes important for biogenesis of the cytoplasmic virion assembly complex.Journal of virology · 2014Article
- Differential properties of cytomegalovirus pUL97 kinase isoforms affect viral replication and maribavir susceptibility.Journal of virology · 2014Article
- Transcription of true late (γ2) cytomegalovirus genes requires UL92 function that is conserved among beta- and gammaherpesviruses.Journal of virology · 2014Article
- Functional annotation of human cytomegalovirus gene products: an update.Frontiers in microbiology · 2014Review
- Cytomegalovirus UL91 is essential for transcription of viral true late (γ2) genes.Journal of virology · 2013Article
- Viral and host control of cytomegalovirus maturation.Trends in microbiology · 2012Review
- Human cytomegalovirus transcriptome activity differs during replication in human fibroblast, epithelial and astrocyte cell lines.The Journal of general virology · 2012Article
- Structural modelling and mutagenesis of human cytomegalovirus alkaline nuclease UL98.The Journal of general virology · 2012Article
- Human cytomegalovirus RL13 gene transcripts in a clinical strain.Virus genes · 2011Article
- Cytomegalovirus pUL96 is critical for the stability of pp150-associated nucleocapsids.Journal of virology · 2011Article
- The human cytomegalovirus gene products essential for late viral gene expression assemble into prereplication complexes before viral DNA replication.Journal of virology · 2011Article
- The human cytomegalovirus UL76 gene regulates the level of expression of the UL77 gene.PloS one · 2010Article
- Proteasome subunits relocalize during human cytomegalovirus infection, and proteasome activity is necessary for efficient viral gene transcription.Journal of virology · 2010Article
- Function of human cytomegalovirus UL97 kinase in viral infection and its inhibition by maribavir.Reviews in medical virology · 2009Review
- Tegument proteins of human cytomegalovirus.Microbiology and molecular biology reviews : MMBR · 2008Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Human cytomegalovirus (HCMV) open reading frames (ORFs) UL93 through UL99 are contained within a region of viral genome that is well conserved in all herpesviruses. Previous reports detailing the expression of ORF UL99 (also referred to as the 28-kDa virion phosphoprotein or pp28) indicated that the pattern of transcription proximal to pp28 is extremely complex and involves a number of large overlapping transcripts, none of which have been characterized. We have used an RNA-mapping approach consisting of Northern (RNA) hybridization, RNase protection, and primer extensions to determine the coding capacity of several large-molecular-weight transcripts which overlap the 1.3- and 1.6-kb UL99-specific transcripts. Our results suggest that six differentially regulated transcripts with sizes of 2.6, 4.7, 5.6, 7.3, 9.1, and 10.5 kb, and derived from the same strand of the viral genome overlap, are 3'-coterminal with the smaller UL99-specific transcripts. On the basis of 5'-end mapping via primer extension and RNase protection, we have determined that the 2.6- to 10.5-kb messages initiate upstream of each of the potential ORFs in this region, UL98, UL97, UL96, UL95, UL94, and UL93. By using cycloheximide and ganciclovir [9-(1,3-dihydroxy-2-propoxymethyl)guanine] to block de novo viral protein synthesis and viral DNA replication, respectively, we have determined that the 2.6-, 4.7-, 5.6-, and 7.3-kb messages have characteristics of early or early-late transcripts, whereas the 9.1- and 10.5-kb messages appear to be true late transcripts. The evolutionary conservation of ORFs UL93 through UL99 and their transcriptional regulation in other herpesviruses are discussed.
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