ArticleThe Journal of physiology1994
Fluo-3 signals associated with potassium contractures in single amphibian muscle fibres.
Article in The Journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Excitation-contraction coupling in mammalian skeletal muscle: Blending old and last-decade research.Frontiers in physiology · 2022Review
- Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera.Scientific reports · 2021Article
- New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin.The Journal of general physiology · 2015Article
- Biomechanics of the sarcolemma and costameres in single skeletal muscle fibers from normal and dystrophin-null mice.Journal of muscle research and cell motility · 2011Article
- A malignant hyperthermia-inducing mutation in RYR1 (R163C): alterations in Ca2+ entry, release, and retrograde signaling to the DHPR.The Journal of general physiology · 2010Article
- Short-term regulation of excitation-contraction coupling by the beta1a subunit in adult mouse skeletal muscle.Biophysical journal · 2005Article
- Inorganic phosphate affects the pCa-force relationship more than the pCa-ATPase by increasing the rate of dissociation of force generating cross-bridges in skinned fibers from both EDL and soleus muscles of the rat.Journal of muscle research and cell motility · 2004Article
- Inactivation of Ca2+ transients in amphibian and mammalian muscle fibres.Journal of muscle research and cell motility · 2004Article
- Differential effects of sarcoplasmic reticular Ca(2+)-ATPase inhibition on charge movements and calcium transients in intact amphibian skeletal muscle fibres.The Journal of physiology · 2002Article
- Fast calcium removal during single twitches in amphibian skeletal muscle fibres.Journal of muscle research and cell motility · 1999Article
- A gap isolation method to investigate electrical and mechanical properties of fully contracting skeletal muscle fibers.Biophysical journal · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The calcium-sensitive dye fluo-3 AM was used to obtain fluorescence signals and calcium transients associated with K+ contractures, twitches and tetani, in intact single muscle fibres of the tropical toad Leptodactylus insularis. 2. The changes in free calcium concentration in the myoplasm ([Ca2+]i) were calculated using the values of the 'off' (k- = 33.5 s-1) and 'on' (k+ = 13.1 microM-1 s-1) rate constants for the binding of calcium to the dye (dissociation constant, Kd = k-/k+). The mean (+/- S.E.M., n = 7) peak [Ca2+]i value during twitches or tetani was 3.9 +/- 0.3 or 4.1 +/- 0.3 microM, respectively, while during maximal K+ contractures, it was 10.3 +/- 0.8 microM. The threshold [Ca2+]i for tension development was about 1 microM. 3. For responses elicited with high [K+]o (80-190 mM), the calcium transients decayed faster than tension. At lower [K+]o (30-70 mM), the decay was slower, and relaxation was complete when [Ca2+]i was still above contractile threshold values. 4. Following a K+ contracture, recovery of the calcium transients associated with twitches occurred before recovery of tension, indicating an apparent dissociation between [Ca2+]i and tension output. This apparent dissociation between calcium and tension output could be attributed to the desensitization of the contractile proteins to calcium, or, more probably, to the non-uniform behaviour of calcium release and/or uptake sites, leading to an unhomogeneous distribution of active sarcomeres along the fibre length and localized sarcomere relaxation.
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