ArticleGenetics1994
REMI-RFLP mapping in the Dictyostelium genome.
Article in Genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Molecular pharmacology in a simple model system: implicating MAP kinase and phosphoinositide signalling in bipolar disorder.Seminars in cell & developmental biology · 2011Review
- Dictyostelium discoideum CenB is a bona fide centrin essential for nuclear architecture and centrosome stability.Eukaryotic cell · 2009Article
- cis-Acting site controlling bidirectional transcription at the growth-differentiation transition in Dictyostelium discoideum.Eukaryotic cell · 2006Article
- Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells.Nucleic acids research · 2001Article
- The cellulose synthase gene of Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1999Review
- Nonhomologous end joining during restriction enzyme-mediated DNA integration in Saccharomyces cerevisiae.Molecular and cellular biology · 1998Article
- Replicon rescue: a novel strategy to clone the genomic DNA flanking insertions of integrating shuttle vector DNA.Nucleic acids research · 1996Article
- Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Analysis of gene function in Dictyostelium.Experientia · 1995Review
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A set of 147 Dictyostelium discoideum strains was constructed by random integration of a vector containing rare restriction sites. The strains were generated by transformation using restriction enzyme-mediated integration (REMI) which results in the integration of linear DNA fragments into randomly distributed genomic restriction sites. Restriction fragment length polymorphism (RFLP) was generated in a single genomic site in each strain. These REMI-RFLP strains were used to confirm gene linkages previously supported by two other physical mapping techniques: yeast artificial chromosome (YAC) contig construction, and megabase-scale restriction mapping. New linkages were uncovered when two or more hybridization probes identified the same RFLP fragments. Probes for 100 genes have marked 53% of the RFLPs, representing greater than 22 Mb of the 40 Mb Dictyostelium genome. Alignment of these and other large fragments along each chromosome should lead to a complete physical map of the Dictyostelium genome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.