ArticleClinical and experimental immunology1995
Cytokine production by normal human monocytes: inter-subject variation and relationship to an IL-1 receptor antagonist (IL-1Ra) gene polymorphism.
Article in Clinical and experimental immunology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers, 5 of them syntheses that pooled it.
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Who cites it
114 citing papers in PubMed, 5 syntheses or guidelines pooled it, 530 citations in OpenAlex.
- TheFrontiers in immunology · 2023Pooled it
- IL-6 and IL-10 gene polymorphisms and cirrhosis of liver risk from a comprehensive analysis.BMC endocrine disorders · 2021Pooled it
- Pooled it
- Association between the IL1B, IL1RN polymorphisms and COPD risk: a meta-analysis.Scientific reports · 2014Pooled it
- Pooled it
- Trial
- A functional synonymous coding variant in the IL1RN gene is associated with survival in septic shock.American journal of respiratory and critical care medicine · 2014Trial
- Evidence of a pharmacogenomic response to interleukin-l receptor antagonist in rheumatoid arthritis.Genes and immunity · 2005Trial
- Association of IL-1beta and IL-1 receptor antagonist haplotypes with rate of decline in lung function in smokers.Thorax · 2001Trial
- Investigating the relationship between the VNTR variant of the interleukin-1 receptor antagonist gene and coronary in-stent restenosis.Molecular biology reports · 2023Article
- Interleukin-1 receptor antagonist: a promising cytokine against human squamous cell carcinomas.Heliyon · 2023Review
- Polymorphisms of TNF-alpha (- 308), IL-1beta (+ 3954) and IL1-Ra (VNTR) are associated to severe stage of endometriosis in Mexican women: a case control study.BMC women's health · 2022Article
- EnterotoxigenicInfection and immunity · 2022Article
- Review
- Association betweenPeerJ · 2021Article
- Genetic Sequence Variants inChildren (Basel, Switzerland) · 2020Article
- External apical root resorption in orthodontic patients: molecular and genetic basis.Journal of family medicine and primary care · 2020Review
- Is clinical effect of autologous conditioned serum in spontaneously occurring equine articular lameness related to ACS cytokine profile?BMC veterinary research · 2020Article
- Interleukin 1 receptor antagonist gene variable number of tandem repeats polymorphism and cutaneous melanoma.Oncology letters · 2019Article
- The association between interleukin-1 polymorphisms and their protein expression in Chinese Han patients with breast cancer.Molecular genetics & genomic medicine · 2019Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monocytes from different individuals show variable cytokine production in response to a variety of stimuli. We wished to determine the sets of conditions (cytokine combinations) that would enable us to demonstrate stable inter-individual differences in the production of IL-1 alpha, IL-1 beta, IL-1Ra, on-6 and tumour necrosis factor-alpha (TNF-alpha) by monocytes. We assessed the ability of a number of recombinant human cytokines (granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon-gamma (IFN-gamma), TNF-alpha, IL-4, IL-6, transforming growth factor-beta (TGF-beta), IL-10 and IL-1Ra)) to stimulate or inhibit the production of one or more of these monocyte products. GM-CSF was found to stimulate the production of all five of these cytokines in a highly reproducible manner. TNF-alpha also up-regulated production of IL-1 alpha, IL-1 beta, IL-1Ra and IL-6 by monocytes, but the variability in the results of cells cultured from the same individuals on different occasions was greater. Other cytokines either stimulated production of only some of the five cytokine products tested, or stimulated the production of some cytokine products while inhibiting production of others. This was especially evident when cytokines were used in combination with GM-CSF: IFN-gamma down-regulated production of IL-1Ra while up-regulating the production of IL-1 alpha/beta, IL-6 and TNF-alpha, while IL-4 had the exact opposite effect. Polymorphisms in regions of cytokine genes that affect transcription may account for some of the interindividual variation in cytokine production. We have shown that a stable estimate of cytokine production phenotype can be obtained when monocytes collected on at least two separate occasions are stimulated by GM-CSF in vitro. We have looked for a relationship between IL-1 production and an 86-bp variable repeat polymorphism in intron 2 of the IL-1Ra gene. A less common allele of this polymorphism (allele 2) was associated with increased production of IL-1Ra protein, and also reduced production of IL-1 alpha protein by monocytes.
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