ArticleCell and tissue research1994
Ultrastructural localization of rDNA and rRNA by in situ hybridization in the nucleolus of human spermatids.
Article in Cell and tissue research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 14 citations in OpenAlex.
- Identification of a novel nucleolin related protein (NRP) gene expressed during rat spermatogenesis.BMC molecular biology · 2009Article
- Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Non-isotopic electron microscope in situ hybridization for studying the functional sub-compartmentalization of the cell nucleus.Histochemistry and cell biology · 1996Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ultrastructural localization of rDNA and rRNA within the nucleolus of human spermatids was investigated by in situ hybridization at steps 1 and 2. Two different digoxigenin-labeled human probes from the rRNA transcription unit were used. Identification of hybrids was performed with immunogold techniques. Comparative observations in the Sertoli cell nucleolus as controls revealed that rDNA was predominantly visualized in the threads of the dense fibrillar component, while rRNA was detected over both the fibrillar component and the granular component. Within the nucleolus of round spermatids in the same sections of seminiferous tubules, rDNA labeling was localized over the spherical or stranded dense fibrillar components. rRNA labeling was found not only over these components but also in the adjacent nucleoplasm rich in ribonucleoprotein particles. These results are consistent with the view that the round spermatid nucleolus is transcriptionally active.
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Registered trials
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