ArticleMammalian genome : official journal of the International Mammalian Genome Society1994
Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): generation of a 5-cM, > 5 megabase contig on mouse chromosome 1.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Article
- Advanced integrated mouse YAC map including BAC framework.Genome research · 2001Article
- Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels.Nucleic acids research · 2000Article
- Characterization of an abundant short interspersed nuclear element (SINE) present in Canis familiaris.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Isolation of monochromosomal hybrids for mouse chromosomes 3, 6, 10, 12, 14, and 18.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Isolation and mapping of three STSs on mouse chromosome 19.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Generation and mapping of Mus spretus strain-specific markers for rapid genomic scanning.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1995Article
- Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, > 5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order to the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ-gld and M. spretus. Three probes were mapped on a [(C3H/HeJ-gld x M. spretus) x C3H/HeJ-gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region.
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