ArticleThe Biochemical journal1995
Translocation of nucleophosmin from nucleoli to nucleoplasm requires ATP.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
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- The pathophysiology of neurodegenerative disease: Disturbing the balance between phase separation and irreversible aggregation.Progress in molecular biology and translational science · 2020Review
- Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation.BMC complementary and alternative medicine · 2012Article
- Nucleolar changes and fibrillarin redistribution following apatone treatment of human bladder carcinoma cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Immunodetection of nucleolar proteins and ultrastructure of nucleoli of soybean root meristematic cells treated with chilling stress and after recovery.Protoplasma · 2009Article
- Inflammation and endothelial dysfunction during aging: role of NF-kappaB.Journal of applied physiology (Bethesda, Md. : 1985) · 2008Review
- Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition.Molecular biology of the cell · 2005Article
- Reconstitution of HIV-1 rev nuclear export: independent requirements for nuclear import and export.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Evidence for the ability of nucleophosmin/B23 to bind ATP.The Biochemical journal · 1998Article
- Isolation and characterization of the human nucleophosmin/B23 (NPM) gene: identification of the YY1 binding site at the 5' enhancer region.Nucleic acids research · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The movement of nucleophosmin from nucleoli to nucleoplasm in HeLa cells induced by cytotoxic drugs and detected by immunofluorescence is inhibited by concomitant treatment with antimycin A in glucose-free medium. Incubation of HeLa cells with antimycin A (300 nM; 30 min) and glucose-free medium resulted in an approximately 90% decrease in cellular ATP pools. To study the biochemical events involved in nucleophosmin translocation, we used an in vitro system consisting of Triton-permeabilized HeLA cells. Incubation of permeabilized cells with ATP (0.5 mM; 1 h) resulted in the translocation of nucleophosmin from nucleoli to nucleoplasm and cytoplasm. Similarly to drug-induced nucleophosmin translocation in whole cultured cells, there is no reduction (measured by e.l.i.s.a.) or degradation of nucleophosmin or change in the ratio of the high-molecular-mass form to the monomeric form (ascertained by Western blotting) during ATP treatment of permeabilized cells. Together, these results indicate a requirement for ATP for redistribution of nucleophosmin from nucleoli to nucleoplasm. Because this permeabilized cell model is simple and efficient and works effectively with exogenous factors, it should provide a powerful tool for investigating the biochemical features of nucleophosmin translocation from nucleoli to nucleoplasm.
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