ArticleProceedings of the National Academy of Sciences of the United States of America1995
Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Use of gene-editing technology to introduce targeted modifications in pigs.Journal of animal science and biotechnology · 2018Review
- Repair of DNA lesions associated with triplex-forming oligonucleotides.Molecular carcinogenesis · 2009Review
- Article
- In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination.Nucleic acids research · 2005Article
- Targeted gene correction by small single-stranded oligonucleotides in mammalian cells.Gene therapy · 2001Article
- Stimulation of homologous recombination through targeted cleavage by chimeric nucleases.Molecular and cellular biology · 2001Article
- A sensitive and rapid assay for homologous recombination in mosquito cells: impact of vector topology and implications for gene targeting.BMC genetics · 2001Article
- The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts.Nucleic acids research · 2000Article
- High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Triple-helix formation induces recombination in mammalian cells via a nucleotide excision repair-dependent pathway.Molecular and cellular biology · 2000Article
- Interstitial deletions and intrachromosomal amplification initiated from a double-strand break targeted to a mammalian chromosome.The EMBO journal · 1998Article
- Processing of targeted psoralen cross-links in Xenopus oocytes.Molecular and cellular biology · 1997Article
- Repair of site-specific double-strand breaks in a mammalian chromosome by homologous and illegitimate recombination.Molecular and cellular biology · 1997Article
- Recombination induced by triple-helix-targeted DNA damage in mammalian cells.Molecular and cellular biology · 1996Article
- Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Targeted gene correction of episomal DNA in mammalian cells mediated by a chimeric RNA.DNA oligonucleotide.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination in gene targeting in most organisms occurs by an inefficient mechanism. Inducing a double-strand break in the chromosomal target may increase this efficiency by allowing recombination to proceed by the highly efficient single-strand annealing mechanism. A gene targeting experiment was modeled in Xenopus oocytes by using a circular plasmid to mimic the chromosomal target site and a homologous linear molecule (pick-up fragment or PUF) as an analogue of the vector DNA. When those two molecules were simply injected together, no recombination was observed. In contrast, when the circular plasmid was cleaved in vivo by injection of the site-specific endonuclease, I-Sce I, relatively efficient intermolecular recombination occurred, involving up to 17% of the cleaved molecules. Recombination was dependent on the stability of the PUF; product yield was increased by using longer fragments and by injecting larger amounts of linear DNA, both of which increased the lifetime of the PUF in the oocytes. These results demonstrate that in vivo double-strand breaks can induce homologous recombination of reluctant substrates and may be useful in augmenting the efficiency of gene targeting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.