ArticleProceedings of the National Academy of Sciences of the United States of America1995
Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 231 citations in OpenAlex.
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- Tractable mammalian cell infections with protozoan-primed bacteria.Journal of visualized experiments : JoVE · 2013Article
- Screening Helicobacter pylori genes induced during infection of mouse stomachs.World journal of gastroenterology · 2012Article
- Identification of Avian pathogenic Escherichia coli genes that are induced in vivo during infection in chickens.Applied and environmental microbiology · 2012Article
- The Fur regulon in anaerobically grown Salmonella enterica sv. Typhimurium: identification of new Fur targets.BMC microbiology · 2011Article
- Identification and characterization of novel and potent transcription promoters of Francisella tularensis.Applied and environmental microbiology · 2011Article
- A novel cdsAB operon is involved in the uptake of L-cysteine and participates in the pathogenesis of Yersinia ruckeri.Journal of bacteriology · 2011Article
- Interaction of Salmonella spp. with the Intestinal Microbiota.Frontiers in microbiology · 2011Article
- Identification of a novel virulence-related gene in Streptococcus suis type 2 strains.Current microbiology · 2010Article
- Screening of in vivo activated genes in Enterococcus faecalis during insect and mouse infections and growth in urine.PloS one · 2010Article
- Novel genetic tools for studying food-borne Salmonella.Current opinion in biotechnology · 2009Article
- A chromosomally located traHIJKCLMN operon encoding a putative type IV secretion system is involved in the virulence of Yersinia ruckeri.Applied and environmental microbiology · 2009Article
- Regulation of fatty acid metabolism by FadR is essential for Vibrio vulnificus to cause infection of mice.Journal of bacteriology · 2008Article
- Genetics-squared: combining host and pathogen genetics in the analysis of innate immunity and bacterial virulence.Immunogenetics · 2007Review
- Identification of an essential gene of Listeria monocytogenes involved in teichoic acid biogenesis.Journal of bacteriology · 2006Article
- A mutation in Flavobacterium psychrophilum tlpB inhibits gliding motility and induces biofilm formation.Applied and environmental microbiology · 2006Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We have recently described a genetic system, termed in vivo expression technology (IVET), that uses an animal as a selective medium to identify genes that pathogenic bacteria specifically express when infecting host tissues. Here, the potential utility of the IVET approach has been expanded with the development of a transcriptional-fusion vector, pIVET8, which uses antibiotics resistance as the basis for selection in host tissues. pIVET8 contains promoterless chloramphenicol acetyltransferase (cat) and lacZY genes. A pool of Salmonella typhimurium clones carrying random cat-lac transcriptional fusions, produced with pIVET8, was used to infect BALB/c mice that were subsequently treated with intraperitoneal injections of chloramphenicol. Strains that survived the selection by expressing the cat gene in the animal were then screened for those that had low-level lacZY expression on laboratory medium. These strains carry operon fusions to genes that are specifically induced in vivo (ivi genes). One of the ivi genes identified (fadB) encodes an enzyme involved in fatty acid oxidation, suggesting that this enzyme might contribute to the metabolism of bactericidal or proinflammatory host fatty acids. The pIVET8-based selection system was also used to identify S. typhimurium genes that are induced in cultured macrophages. The nature of ivi gene products will provide a more complete understanding of the metabolic, physiological, and genetic factors that contribute to the virulence of microbial pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.