ArticleMolecular & general genetics : MGG1994
Sequence of the HAP3 transcription factor of Kluyveromyces lactis predicts the presence of a novel 4-cysteine zinc-finger motif.
Article in Molecular & general genetics : MGG, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 50 citations in OpenAlex.
- Increasing NADPH impairs fungal HBioresources and bioprocessing · 2022Article
- Diverse Hap43-independent functions of the Candida albicans CCAAT-binding complex.Eukaryotic cell · 2013Article
- Identifying cis-regulatory changes involved in the evolution of aerobic fermentation in yeasts.Genome biology and evolution · 2013Article
- Kluyveromyces lactis: a suitable yeast model to study cellular defense mechanisms against hypoxia-induced oxidative stress.Oxidative medicine and cellular longevity · 2012Review
- Transcriptional regulatory elements in fungal secondary metabolism.Journal of microbiology (Seoul, Korea) · 2011Review
- A stress response related to the carbon source and the absence of KlHAP2 in Kluyveromyces lactis.Journal of industrial microbiology & biotechnology · 2011Article
- The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes.Nucleic acids research · 2010Article
- Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron.The EMBO journal · 2007Article
- Functional characterization of the Frt1 sugar transporter and of fructose uptake in Kluyveromyces lactis.Current genetics · 2003Article
- The S. Cerevisiae HAP complex, a key regulator of mitochondrial function, coordinates nuclear and mitochondrial gene expression.Comparative and functional genomics · 2003Article
- Article
- Mutations affecting a yeast mitochondrial inner membrane protein, pnt1p, block export of a mitochondrially synthesized fusion protein from the matrix.Molecular and cellular biology · 1999Article
- Yeast carbon catabolite repression.Microbiology and molecular biology reviews : MMBR · 1998Review
- A survey of 178 NF-Y binding CCAAT boxes.Nucleic acids research · 1998Review
- The hapC gene of Aspergillus nidulans is involved in the expression of CCAAT-containing promoters.Molecular & general genetics : MGG · 1996Article
- Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis.Current genetics · 1995Article
- Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable, despite low overall homology.Current genetics · 1994Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The Kluyveromyces lactis homologue of the Saccharomyces cerevisiae HAP3 gene was isolated by functional complementation of the respiratory-deficient phenotype of the S. cerevisiae hap3::HIS4 strain SHY40. The KlHAP3 gene encodes a protein of 205 amino acids, of which the central B-domain of 90 residues is highly homologous to HAP3 counterparts of S. cerevisiae and higher eukaryotes. The protein contains a novel 4-cysteine zinc-finger motif and we propose by analogy that all other homologous HAP3 proteins contain the same motif, with the position containing the third cysteine being occupied by a serine residue. In contrast to the situation in S. cerevisiae, disruption of the KlHAP3 gene in K. lactis does not result in a respiratory-deficient phenotype and the growth of the null strain is indistinguishable from wild type. There is also no effect on the expression of the carbon source-regulated KlCYC1 gene, suggesting either a different role for the HAP2/3/4 complex, or the existence of a different mechanism of carbon source regulation. Sequence verification of the S. cerevisiae HAP3 locus reveals that, just as in K. lactis, a long open reading frame (ORF) is present upstream of the HAP3 gene. These highly homologous ORFs are predicted to have at least eight membrane-spanning fragments, but do not show significant homology to any known sequence present in databases. The ScORFX gene is transcribed in the opposite direction to ScHAP3, but, in contrast to an earlier report by Hahn et al. (1988), the transcripts of the two genes do not overlap. The model proposed by these authors, in which the ScHAP3 gene is regulated by an anti-sense non-coding mRNA, is therefore not correct.
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