ArticleThe Journal of cell biology1995
Both synchronous and asynchronous muscle isoforms of projectin (the Drosophila bent locus product) contain functional kinase domains.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging.Skeletal muscle · 2025Article
- Twitchin kinase inhibits muscle activity.Molecular biology of the cell · 2017Article
- Elastic proteins in the flight muscle of Manduca sexta.Archives of biochemistry and biophysics · 2015Article
- A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila.G3 (Bethesda, Md.) · 2014Article
- The myofibrillar protein, projectin, is highly conserved across insect evolution except for its PEVK domain.Journal of molecular evolution · 2008Article
- Protein expression in a Drosophila model of Parkinson's disease.Journal of proteome research · 2007Article
- The structural role of high molecular weight tropomyosins in dipteran indirect flight muscle and the effect of phosphorylation.Journal of muscle research and cell motility · 2006Article
- Muscle atrophy in titin M-line deficient mice.Journal of muscle research and cell motility · 2005Article
- In indirect flight muscles Drosophila projectin has a short PEVK domain, and its NH2-terminus is embedded at the Z-band.Journal of muscle research and cell motility · 2005Article
- Assembly of the giant protein projectin during myofibrillogenesis in Drosophila indirect flight muscles.BMC cell biology · 2004Article
- DIM-1, a novel immunoglobulin superfamily protein in Caenorhabditis elegans, is necessary for maintaining bodywall muscle integrity.Genetics · 2003Article
- The entire cDNA sequences of projectin isoforms of crayfish claw closer and flexor muscles and their localization.Journal of muscle research and cell motility · 2003Article
- D-Titin: a giant protein with dual roles in chromosomes and muscles.The Journal of cell biology · 2000Article
- Requirements of Kettin, a giant muscle protein highly conserved in overall structure in evolution, for normal muscle function, viability, and flight activity of Drosophila.The Journal of cell biology · 2000Article
- The Drosophila projectin mutant, bentD, has reduced stretch activation and altered indirect flight muscle kinetics.Journal of muscle research and cell motility · 1999Article
- Human autoantibodies reveal titin as a chromosomal protein.The Journal of cell biology · 1998Article
- Giant protein kinases: domain interactions and structural basis of autoregulation.The EMBO journal · 1996Article
- The Caenorhabditis elegans gene unc-89, required fpr muscle M-line assembly, encodes a giant modular protein composed of Ig and signal transduction domains.The Journal of cell biology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In Drosophila, the large muscle protein, projectin, has very different localizations in synchronous and asynchronous muscles, suggesting that projectin has different functions in different muscle types. The multiple projectin isoforms are encoded by a single gene; however they differ significantly in size (as detected by gel mobility) and show differences in some peptide fragments, presumably indicating alternative splicing or termination. We now report additional sequence of the projectin gene, showing a kinase domain and flanking regions highly similar to equivalent regions of twitchin, including a possible autoinhibitory region. In spite of apparent differences in function, all isoforms of projectin have the kinase domain and all are capable of autophosphorylation in vitro. The projectin gene is in polytene region 102C/D where the bentD phenotype maps. The recessive lethality of bentD is associated with a breakpoint that removes sequence of the projectin kinase domain. We find that different alleles of the highly mutable recessive lethal complementation group, l(4)2, also have defects in different parts of the projectin sequence, both NH2-terminal and COOH-terminal to the bentD breakpoint. These alleles are therefore renamed as alleles of the bent locus. Adults heterozygous for projectin mutations show little, if any, effect of one defective gene copy, but homozygosity for any of the defects is lethal. The times of death can vary with allele. Some alleles kill the embryos, others are larval lethal. These molecular studies begin to explain why genetic studies suggested that l(4)2 was a complex (or pseudoallelic) locus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.