ArticleThe Journal of cell biology1995
Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 84 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
84 citing papers in PubMed, 239 citations in OpenAlex.
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- Network organisation and the dynamics of tubules in the endoplasmic reticulum.Scientific reports · 2021Article
- The Mechanics and Thermodynamics of Tubule Formation in Biological Membranes.The Journal of membrane biology · 2021Review
- Kinesin-1 activity recorded in living cells with a precipitating dye.Nature communications · 2021Article
- Photoactivatable Hsp47: A Tool to Regulate Collagen Secretion and Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- Intermediate compartment (IC): from pre-Golgi vacuoles to a semi-autonomous membrane system.Histochemistry and cell biology · 2018Review
- Tau Proteins and Tauopathies in Alzheimer's Disease.Cellular and molecular neurobiology · 2018Review
- The role of the cytoskeleton in biomineralisation in haptophyte algae.Scientific reports · 2017Article
- Consecutive entosis stages in human substrate-dependent cultured cells.Scientific reports · 2017Article
- Hippocampal to basal forebrain transport of MnNeuroImage · 2017Article
- CNV Analysis Associates AKNAD1 with Type-2 Diabetes in Jordan Subpopulations.Scientific reports · 2015Article
- A palette of fluorescent proteins optimized for diverse cellular environments.Nature communications · 2015Article
- Huntingtin is required for ER-to-Golgi transport and for secretory vesicle fusion at the plasma membrane.Disease models & mechanisms · 2014Article
- Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration.The Journal of cell biology · 2014Article
- Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport.Biology open · 2014Article
- Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development.Gene expression patterns : GEP · 2013Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The distribution and dynamics of both the ER and Golgi complex in animal cells are known to be dependent on microtubules; in many cell types the ER extends toward the plus ends of microtubules at the cell periphery and the Golgi clusters at the minus ends of microtubules near the centrosome. In this study we provide evidence that the microtubule motor, kinesin, is present on membranes cycling between the ER and Golgi and powers peripherally directed movements of membrane within this system. Immunolocalization of kinesin at both the light and electron microscopy levels in NRK cells using the H1 monoclonal antibody to kinesin heavy chain, revealed kinesin to be associated with all membranes of the ER/Golgi system. At steady-state at 37 degrees C, however, kinesin was most concentrated on peripherally distributed, pre-Golgi structures containing beta COP and vesicular stomatitis virus glycoprotein newly released from the ER. Upon temperature reduction or nocodazole treatment, kinesin's distribution shifted onto the Golgi, while with brefeldin A (BFA)-treatment, kinesin could be found in both Golgi-derived tubules and in the ER. This suggested that kinesin associates with membranes that constitutively cycle between the ER and Golgi. Kinesin's role on these membranes was examined by microinjecting kinesin antibody. Golgi-to-ER but not ER-to-Golgi membrane transport was found to be inhibited by the microinjected anti-kinesin, suggesting kinesin powers the microtubule plus end-directed recycling of membrane to the ER, and remains inactive on pre-Golgi intermediates that move toward the Golgi complex.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.