ArticleIn vitro cellular & developmental biology. Animal1994
Genital tract growth factors from a moth, the tobacco budworm, Heliothis virescens.
Article in In vitro cellular & developmental biology. Animal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 7 citations in OpenAlex.
- From Mosquito Ovaries to Ecdysone; from Ecdysone toInsects · 2022Review
- Host-parasitoid interactions relating to penetration of the whitefly, Bemisia tabaci, by the parasitoid wasp, Eretmocerus mundus.Journal of insect science (Online) · 2005Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development and maturation of the genital tract of the moth, Heliothis virescens, takes place within a few days in the pupal stage. The insect steroid hormone, 20-hydroxyecdysone (20E), essential during this period, stimulated testis sheath and fat body tissue to secrete factors that, in turn, stimulated growth and development of pupal spermducts and genital imaginal discs in vitro. Factors could be extracted in aqueous solution from tissues incubated for 24 h in media containing 1000pg/microliters 20E, and partially purified by chromatography on polyacrylamide gels. Ten active molecular weight fractions were separated from testis sheath extracts, and 9 from fat body extracts. Most fractions were labile to protease, although the activity of six of the fractions was also destroyed by lipase. Testis sheath, fat body tissue, and active fractions, caused partial development of the genital tract in vitro, as well as increased incorporation of [3H]methionine into precipitable protein and [3H]thymidine into nuclear material.
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Registered trials
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