ArticlePflugers Archiv : European journal of physiology1994
cAMP-dependent activation of ion conductances in bronchial epithelial cells.
Article in Pflugers Archiv : European journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Effect of apical chloride concentration on the measurement of responses to CFTR modulation in airway epithelia cultured from nasal brushings.Physiological reports · 2020Article
- CaCellular and molecular life sciences : CMLS · 2017Article
- Patch clamp on the luminal membrane of exocrine gland acini from frog skin (Rana esculenta) reveals the presence of cystic fibrosis transmembrane conductance regulator-like Cl- channels activated by cyclic AMP.The Journal of general physiology · 1998Article
- Heterogeneity of chloride channels in the apical membrane of isolated mitochondria-rich cells from toad skin.The Journal of general physiology · 1996Article
- Regulation of epithelial ion channels by the cystic fibrosis transmembrane conductance regulator.Journal of molecular medicine (Berlin, Germany) · 1996Review
- The membrane transporters regulating epithelial NaCl secretion.Pflugers Archiv : European journal of physiology · 1996Review
- Clusters of Cl- channels in CFTR-expressing Sf9 cells switch spontaneously between slow and fast gating modes.Pflugers Archiv : European journal of physiology · 1996Article
- Culture-dependent expression of Na+ conductances in airway epithelial cells.Pflugers Archiv : European journal of physiology · 1996Article
- N-Acetyl-L-cysteine and its derivatives activate a Cl- conductance in epithelial cells.Pflugers Archiv : European journal of physiology · 1996Article
- Actin-dependent activation of ion conductances in bronchial epithelial cells.Pflugers Archiv : European journal of physiology · 1995Article
- cAMP-dependent activation of ion conductances in bronchial epithelial cells.Pflugers Archiv : European journal of physiology · 1994Article
- Ca(2+)- and swelling-induced activation of ion conductances in bronchial epithelial cells.Pflugers Archiv : European journal of physiology · 1994Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cAMP-dependent activation of Cl- channels was studied in a bronchial epithelial cell line (16HBE14o-) in fast and slow whole-cell, and cell-attached patch-clamp experiments. The cells are known to express high levels of cystic fibrosis transmembrane conductance regulator mRNA and protein. Isoproterenol, forskolin and histamine (all 10 mumol/l) reversibly and significantly depolarized the membrane voltage (Vm) and increased the whole-cell Cl- conductance significantly by 34.0 +/- 0.9 (n = 3), 18.1 +/- 2.7 (n = 50), and 25 +/- 4.5 (n = 37) nS respectively. The effect of histamine was blocked by cimetidine (10 mumol, n = 5) but not by diphenhydramine (10 mumol/l, n = 4), which suggests binding of histamine to H2 receptors. The forskolin-induced current was not inhibited significantly by 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (0.5 mmol/l, n = 9) nor glibenclamide (10 mumol/l, n = 3) and had an anion-permeability sequence of Cl = Br- > I- (n = 9). In cell-attached recordings forskolin (10 mumol/l) increased the conductance of the patched membrane from 65.5 +/- 13.6 pS to 150.8 +/- 33.2 pS (n = 30). Although the conductance was increased significantly, clear ion channel events occurring in parallel with the current activation were not detected in the cell-attached membrane. In 4 out of 30 cell-attached recordings single-channel currents were observed. These channels, with a single-channel conductance of about 6 pS, were already active before forskolin was added. No effect of forskolin on the channel amplitude, open probability or kinetics of these channels was observed.(ABSTRACT TRUNCATED AT 250 WORDS)
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.