ArticlePflugers Archiv : European journal of physiology1994
Ion-transporting activity in the murine colonic epithelium of normal animals and animals with cystic fibrosis.
Article in Pflugers Archiv : European journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 62 citations in OpenAlex.
- Status of fluid and electrolyte absorption in cystic fibrosis.Cold Spring Harbor perspectives in medicine · 2013Article
- Bestrophin-2 mediates bicarbonate transport by goblet cells in mouse colon.The Journal of clinical investigation · 2010Article
- Secretagogue stimulation enhances NBCe1 (electrogenic Na(+)/HCO(3)(-) cotransporter) surface expression in murine colonic crypts.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Characterisation of chloride currents across the proximal colon in CftrTgH(neoim)1Hgu congenic mice.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
- Expression and regulation of the Na+-K+-2Cl- cotransporter NKCC1 in the normal and CFTR-deficient murine colon.The Journal of physiology · 2003Article
- GFP-tagged CFTR transgene is functional in the G551D cystic fibrosis mouse colon.The Journal of membrane biology · 2003Article
- Dextran sodium sulphate-induced colitis perturbs muscarinic cholinergic control of colonic epithelial ion transport.British journal of pharmacology · 2002Article
- Phenanthrolines--a new class of CFTR chloride channel openers.British journal of pharmacology · 2001Article
- Assessment of CFTR chloride channel openers in intact normal and cystic fibrosis murine epithelia.British journal of pharmacology · 2001Article
- Formal analysis of electrogenic sodium, potassium, chloride and bicarbonate transport in mouse colon epithelium.British journal of pharmacology · 1999Article
- Importance of basolateral K+ conductance in maintaining Cl- secretion in murine nasal and colonic epithelia.The Journal of physiology · 1998Article
- A functional CFTR protein is required for mouse intestinal cAMP-, cGMP- and Ca(2+)-dependent HCO3- secretion.The Journal of physiology · 1997Article
- Complementation of null CF mice with a human CFTR YAC transgene.The EMBO journal · 1997Article
- Chloride secretion in the trachea of null cystic fibrosis mice: the effects of transfection with pTrial10-CFTR2.The Journal of physiology · 1997Article
- Isolation and long-term culture of gallbladder epithelial cells from wild-type and CF mice.In vitro cellular & developmental biology. Animal · 1997Article
- Ion Transport in Health and Disease. Symposium proceedings. University College Cork, 19-20 September 1995.The Journal of physiology · 1995Article
- A mouse model for the cystic fibrosis delta F508 mutation.The EMBO journal · 1995Article
- The genetic advantage hypothesis in cystic fibrosis heterozygotes: a murine study.The Journal of physiology · 1995Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Electrogenic ion transport in the isolated colonic epithelium from normal and transgenic mice with cystic fibrosis (CF mice) has been investigated under short-circuit current (Isc) conditions. Normal tissues showed chloride secretion in response to carbachol or forskolin, which was sensitive to the Na-K-2Cl cotransport inhibitor, frusemide. Responses to both agents were maintained for at least 12 h in vitro, but the responses to carbachol changed in format throughout this period. By contrast CF colons failed to show the normal secretory responses to carbachol and forskolin, most preparations showing a decrease in Isc that was immediately reversed by frusemide. In CF colons addition of Ba2+ ions or tetraethylammonium (TEA+) to the apical bathing solution antagonised the reduction in Isc caused by the secretagogues. It is concluded that the reduction in Isc in CF colons is due to electrogenic K+ secretion and this was confirmed by flux studies using rubidium-86. In normal colons exposed to TEA+ the responses to forskolin were greater, but not significantly so, presumably because the minor K(+)-secretory responses are dominated by major chloride-secretory responses. Again rubidium-86 fluxes showed an increase of K+ secretion in normal colons receiving forskolin. Since the amiloride-sensitive current was not different in CF and normal colons there was no evidence that the CF mice were stressed in a way that increased mineralocorticoid levels and hence K+ secretion. Knowledge of the phenotype of the colonic epithelium of the CF mouse sets the baseline from which attempts at gene therapy for the gut must be judged.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.