ArticleJournal of bacteriology1995
Analysis of the Mycobacterium tuberculosis 85A antigen promoter region.
Article in Journal of bacteriology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 72 citations in OpenAlex.
- Mycobacterium tuberculosis Transcription Machinery: Ready To Respond to Host Attacks.Journal of bacteriology · 2016Review
- Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults.Journal of immunology research · 2015Article
- A two-step strategy for the complementation of M. tuberculosis mutants.Genetics and molecular biology · 2011Article
- Synthesis and biological evaluation of NAS-21 and NAS-91 analogues as potential inhibitors of the mycobacterial FAS-II dehydratase enzyme Rv0636.Microbiology (Reading, England) · 2008Article
- Expression of the Mycobacterium tuberculosis acr-coregulated genes from the DevR (DosR) regulon is controlled by multiple levels of regulation.Infection and immunity · 2008Article
- Unique mechanism of action of the thiourea drug isoxyl on Mycobacterium tuberculosis.The Journal of biological chemistry · 2003Article
- Identification of a regulated alkaline phosphatase, a cell surface-associated lipoprotein, in Mycobacterium smegmatis.Journal of bacteriology · 2003Article
- Immune response induced by recombinant Mycobacterium bovis BCG producing the cholera toxin B subunit.Infection and immunity · 2003Article
- Regulation of expression of mas and fadD28, two genes involved in production of dimycocerosyl phthiocerol, a virulence factor of Mycobacterium tuberculosis.Journal of bacteriology · 2002Article
- Mycobacterium bovis BCG producing interleukin-18 increases antigen-specific gamma interferon production in mice.Infection and immunity · 2002Article
- Characterization of a putative alpha-mannosyltransferase involved in phosphatidylinositol trimannoside biosynthesis in Mycobacterium tuberculosis.The Biochemical journal · 2002Article
- Humoral and cellular immune responses in mice immunized with recombinant Mycobacterium bovis Bacillus Calmette-Guérin producing a pertussis toxin-tetanus toxin hybrid protein.Infection and immunity · 1999Article
- Systemic and mucosal immune responses after intranasal administration of recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing glutathione S-transferase from Schistosoma haematobium.Infection and immunity · 1998Article
- mmr, a Mycobacterium tuberculosis gene conferring resistance to small cationic dyes and inhibitors.Journal of bacteriology · 1998Article
- A crtB homolog essential for photochromogenicity in Mycobacterium marinum: isolation, characterization, and gene disruption via homologous recombination.Journal of bacteriology · 1997Article
- High-level heterologous expression and secretion in rapidly growing nonpathogenic mycobacteria of four major Mycobacterium tuberculosis extracellular proteins considered to be leading vaccine candidates and drug targets.Infection and immunity · 1997Article
- Open reading frame 3, which is adjacent to the mycocerosic acid synthase gene, is expressed as an acyl coenzyme A synthase in Mycobacterium bovis BCG.Journal of bacteriology · 1997Article
- Mycobacterium tuberculosis efpA encodes an efflux protein of the QacA transporter family.Clinical and diagnostic laboratory immunology · 1997Article
- Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis.Infection and immunity · 1996Article
- A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements.Journal of bacteriology · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mycobacterial expression-secretion vector was constructed in which the Escherichia coli alkaline phosphatase (phoA) reporter gene was placed under the control of the Mycobacterium tuberculosis 85A promoter and secretion signal sequences. In recombinant Mycobacterium smegmatis and Mycobacterium bovis BCG, PhoA activity could readily be detected on the mycobacterial cell surface and in the culture supernatant, indicating that the 85A signals can drive heterologous expression and secretion in both species. In contrast to the mycobacteria, the 85A promoter did not function in E. coli. We mapped the promoter region by progressive deletions using BAL 31 exonuclease and by primer extension analysis. Insertion and deletion mutations within the promoter region indicated that, unlike most E. coli promoters but similar to Streptomyces promoters, the position of the putative -35 region was not critical for efficient promoter activity. In addition, we investigated the ability of the identified signals to drive the production and secretion in BCG of recombinant Schistosoma mansoni glutathione S-transferase (Sm28GST), a protective antigen against schistosomiasis. BALB/c mice immunized with the recombinant BCG by a single dose exhibited a weak but specific T-cell response to Sm28GST.
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