ArticleThe EMBO journal1995
The bovine papillomavirus 1 E2 protein contains two activation domains: one that interacts with TBP and another that functions after TBP binding.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 74 citations in OpenAlex.
- Identification and characterization of a novel papillomavirus in thornback skate (Microbial genomics · 2025Article
- Type-specific interaction between human papillomavirus type 58 E2 protein and E7 protein inhibits E7-mediated oncogenicity.The Journal of general virology · 2012Article
- HPV16 E2 could act as down-regulator in cellular genes implicated in apoptosis, proliferation and cell differentiation.Virology journal · 2011Article
- Small molecule inhibitors of human papillomavirus protein - protein interactions.The open virology journal · 2011Article
- TGF-b2 induction regulates invasiveness of Theileria-transformed leukocytes and disease susceptibility.PLoS pathogens · 2010Article
- Modification of papillomavirus E2 proteins by the small ubiquitin-like modifier family members (SUMOs).Virology · 2008Article
- Interaction of papillomavirus E2 protein with the Brm chromatin remodeling complex leads to enhanced transcriptional activation.Journal of virology · 2007Article
- Association of bovine papillomavirus E2 protein with nuclear structures in vivo.Journal of virology · 2005Article
- Interaction of bovine papillomavirus E2 protein with Brd4 stabilizes its association with chromatin.Journal of virology · 2005Article
- Analysis of chromatin attachment and partitioning functions of bovine papillomavirus type 1 E2 protein.Journal of virology · 2004Article
- UVB irradiation reduces the half-life and transactivation potential of the human papillomavirus 16 E2 protein.Oncogene · 2003Article
- The Papillomavirus E2 protein binds to and synergizes with C/EBP factors involved in keratinocyte differentiation.Journal of virology · 2003Article
- Human TATA binding protein inhibits human papillomavirus type 11 DNA replication by antagonizing E1-E2 protein complex formation on the viral origin of replication.Journal of virology · 2002Article
- Variable oncogene promoter activity of human papillomavirus type 16 cervical cancer isolates from Australia.Journal of clinical microbiology · 2001Article
- The E8 domain confers a novel long-distance transcriptional repression activity on the E8E2C protein of high-risk human papillomavirus type 31.Journal of virology · 2001Article
- Crystal structure of the Lrp-like transcriptional regulator from the archaeon Pyrococcus furiosus.The EMBO journal · 2001Article
- Herpes simplex virus type 1 ICP4 promotes transcription preinitiation complex formation by enhancing the binding of TFIID to DNA.Journal of virology · 2000Article
- Adeno-associated virus type 2 rep protein inhibits human papillomavirus type 16 E2 recruitment of the transcriptional coactivator p300.Journal of virology · 2000Article
- Mechanisms of human papillomavirus E2-mediated repression of viral oncogene expression and cervical cancer cell growth inhibition.Journal of virology · 2000Article
- Alleviation of human papillomavirus E2-mediated transcriptional repression via formation of a TATA binding protein (or TFIID)-TFIIB-RNA polymerase II-TFIIF preinitiation complex.Molecular and cellular biology · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The E2 transactivator of bovine papillomavirus type-1 is unable to activate minimal promoters in vivo that contain only E2 binding sites and a TATA box. This block can be overcome by over-expression of human TATA binding protein (TBP) or by the addition of either SP1 binding sites or an initiator element to the promoter, suggesting that the binding of TFIID may normally be a rate-limiting step for activation by E2. Surprisingly, purified E2 and TBP bind co-operatively to DNA in vitro when the sites are closely spaced. E2 does not affect the on rate of association but reduces the off rate. The E2 region responsible for this effect is located in the hinge region that links the classic transactivation and DNA binding domains. We demonstrate that the TBP stabilizing domain contributes in vivo to co-operativity with co-expressed TBP and to activation of the major late minimal promoter (MLP) containing E2 sites. In contrast, promoters with SP1 sites are activated to wild-type levels by such a mutant. This promoter specificity is also evident in vitro. A truncated E2 mutant, lacking the classic transactivation domain but containing the TBP stabilizing domain, stimulates transcription of the MLP in vitro, but does not activate promoters with SP1 sites. In conclusion, our results show that the E2 transactivation domain has a modular structure. We have identified one domain which probably acts at an early step in the assembly of the pre-initiation complex and which is involved in reducing the dissociation rate of bound TBP in vitro. The classic N-terminal activation domain of E2 might affect one or several step(s) in the assembly of the preinitiation complex occurring after the binding of TFIID.
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