ArticleThe EMBO journal1995
A role for soluble NSF attachment proteins (SNAPs) in regulated exocytosis in adrenal chromaffin cells.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 102 citations in OpenAlex.
- Multiple paralogues of α-SNAP in Giardia lamblia exhibit independent subcellular localization and redistribution during encystation and stress.Parasites & vectors · 2018Article
- Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.PloS one · 2015Article
- The complete genome sequences of poxviruses isolated from a penguin and a pigeon in South Africa and comparison to other sequenced avipoxviruses.BMC genomics · 2014Article
- Syntaxin 31 functions in Glycine max resistance to the plant parasitic nematode Heterodera glycines.Plant molecular biology · 2014Article
- A conserved membrane attachment site in alpha-SNAP facilitates N-ethylmaleimide-sensitive factor (NSF)-driven SNARE complex disassembly.The Journal of biological chemistry · 2009Article
- Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction.PloS one · 2009Article
- Activation of the store-operated calcium current ICRAC can be dissociated from regulated exocytosis in rat basophilic leukaemia (RBL-1) cells.The Journal of physiology · 2003Article
- SGS1 is a multicopy suppressor of srs2: functional overlap between DNA helicases.Nucleic acids research · 2002Article
- Differential regulation of exocytosis by alpha- and beta-SNAPs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Endothelial transcytotic machinery involves supramolecular protein-lipid complexes.Molecular biology of the cell · 2001Article
- The genome of fowlpox virus.Journal of virology · 2000Article
- Comparison of cysteine string protein (Csp) and mutant alpha-SNAP overexpression reveals a role for csp in late steps of membrane fusion in dense-core granule exocytosis in adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells.The EMBO journal · 1999Article
- Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomes.Molecular and cellular biology · 1998Article
- Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis.The Journal of cell biology · 1997Article
- Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis.Molecular biology of the cell · 1997Article
- Membrane fusion protein synexin (annexin VII) as a Ca2+/GTP sensor in exocytotic secretion.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells.The Journal of cell biology · 1996Article
- Domains of alpha-SNAP required for the stimulation of exocytosis and for N-ethylmalemide-sensitive fusion protein (NSF) binding and activation.Molecular biology of the cell · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Digitonin-permeabilized chromaffin cells secrete catecholamines by exocytosis in response to micromolar Ca2+ concentrations, but lose the ability to secrete in response to Ca2+ as the cells lose soluble proteins through the plasma membrane pores. Such secretory run-down can be retarded by cytosolic fractions, thus providing an assay for proteins potentially involved in the exocytotic process. We have used this assay to investigate the role of N-ethylmaleimide-sensitive fusion protein (NSF) and soluble NSF attachment proteins (SNAPs) in regulated exocytosis. Recombinant alpha- and gamma-SNAP stimulated Ca(2+)-dependent exocytosis, although recombinant NSF was ineffective, despite the fact that NSF and alpha-SNAP leak from the permeabilized cells with similar time courses. However, around one third of cellular NSF was found to be present in a non-cytosolic form and so it is possible that this is sufficient for exocytosis and that exogenous SNAPs stimulate the exocytotic mechanism by acting on the leakage-insensitive NSF. The stimulatory effect of alpha-SNAP displayed a biphasic dose-response curve and was maximal at 20 micrograms/ml. The effect of alpha-SNAP was Ca(2+)- and MgATP-dependent and was inhibited by N-ethylmaleimide and botulinum A neurotoxin, indicating a bona fide action on the exocytotic mechanism. Furthermore, Ca2+ concentrations which trigger catecholamine secretion acted to prevent the leakage of NSF and alpha-SNAP from permeabilized cells. These findings provide functional evidence for a role of SNAPs in regulated exocytosis in chromaffin cells.
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