ArticleProceedings of the National Academy of Sciences of the United States of America1995
Visualization of the thyrotropin-releasing hormone receptor and its ligand during endocytosis and recycling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 106 citations in OpenAlex.
- Biochemical and physiological insights into TRH receptor-mediated signaling.Frontiers in cell and developmental biology · 2022Review
- The Thyrotropin-Releasing Hormone-Degrading Ectoenzyme, a Therapeutic Target?Frontiers in pharmacology · 2020Review
- Endocytosis and Trafficking of Natriuretic Peptide Receptor-A: Potential Role of Short Sequence Motifs.Membranes · 2015Review
- Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors.Biochimica et biophysica acta · 2014Review
- Desensitization, trafficking, and resensitization of the pituitary thyrotropin-releasing hormone receptor.Frontiers in neuroscience · 2012Article
- Ligand-mediated endocytosis and intracellular sequestration of guanylyl cyclase/natriuretic peptide receptors: role of GDAY motif.Molecular and cellular biochemistry · 2010Article
- Subcellular trafficking of the TRH receptor: effect of phosphorylation.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Excitation of histaminergic tuberomamillary neurons by thyrotropin-releasing hormone.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Excitatory effects of thyrotropin-releasing hormone in the thalamus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein.The Biochemical journal · 2004Article
- Kinetic analysis of the internalization and recycling of [3H]TRH and C-terminal truncations of the long isoform of the rat thyrotropin-releasing hormone receptor-1.The Biochemical journal · 2000Article
- An acidic cluster in the cytosolic domain of human cytomegalovirus glycoprotein B is a signal for endocytosis from the plasma membrane.Journal of virology · 1999Article
- Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein.British journal of pharmacology · 1999Review
- Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation.The Biochemical journal · 1999Article
- Neuroregulation of ProTRH biosynthesis and processing.Endocrine · 1999Review
- Fates of endocytosed somatostatin sst2 receptors and associated agonists.The Biochemical journal · 1998Article
- A serine cluster prevents recycling of the V2 vasopressin receptor.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Endocytosis and recycling of varicella-zoster virus Fc receptor glycoprotein gE: internalization mediated by a YXXL motif in the cytoplasmic tail.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Endocytosis and recycling of both thyrotropin-releasing hormone (TRH) and its G-protein-coupled receptor were visualized by conventional and confocal fluorescence microscopy in pituitary cells using a rhodamine-labeled TRH analog (Rhod-TRH) and indirect immunofluorescent staining of cells stably transfected with an epitope-tagged TRH receptor (TRHR). The epitope-tagged TRHR was confined to the cell surface prior to agonist treatment. Both Rhod-TRH and TRHR were also localized on the plasma membrane after agonist binding at 0 degrees C. Ligand binding at 37 degrees C resulted in rapid endocytosis, and both Rhod-TRH and the epitope-tagged TRHR appeared in cytoplasmic vesicles within 5 min. Fluorescently labeled TRH and transferrin colocalized in the same endocytotic vesicles, and internalization of Rhod-TRH and TRHR was inhibited by hypertonic medium, suggesting that endocytosis occurred by a clathrin-dependent mechanism. Internalized TRHRs returned to the membrane within 20 min after removal of TRH, and cycloheximide did not block receptor recycling. A mutant TRHR truncated at Cys335 signaled but did not internalize Rhod-TRH, confirming the importance of the carboxyl terminus of the TRHR in receptor-mediated endocytosis. Thus, the TRH-TRHR complex is endocytosed via clathrin-coated vesicles and the receptor is recycled to the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.