ArticleJournal of clinical pathology1994
Which proliferation markers for routine immunohistology? A comparison of five antibodies.
Article in Journal of clinical pathology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Antitumor and antiangiogenic activity of the novel chimeric inhibitor animacroxam in testicular germ cell cancer.Molecular oncology · 2019Article
- Subicular hypotrophy in fetuses with Down syndrome and in the Ts65Dn model of Down syndrome.Brain pathology (Zurich, Switzerland) · 2019Article
- Excitotoxic cell death induces delayed proliferation of endogenous neuroprogenitor cells in organotypic slice cultures of the rat spinal cord.Cell death & disease · 2013Article
- Widespread proliferation impairment and hypocellularity in the cerebellum of fetuses with down syndrome.Brain pathology (Zurich, Switzerland) · 2011Article
- Environmental enrichment rescues postnatal neurogenesis defect in the male and female Ts65Dn mouse model of Down syndrome.Developmental neuroscience · 2011Article
- The COX-2 inhibitors, meloxicam and nimesulide, suppress neurogenesis in the adult mouse brain.British journal of pharmacology · 2010Article
- Lithium restores neurogenesis in the subventricular zone of the Ts65Dn mouse, a model for Down syndrome.Brain pathology (Zurich, Switzerland) · 2010Article
- Downregulation of both p21/Cip1 and p27/Kip1 produces a more aggressive prostate cancer phenotype.Cell cycle (Georgetown, Tex.) · 2008Article
- Neurogenesis impairment and increased cell death reduce total neuron number in the hippocampal region of fetuses with Down syndrome.Brain pathology (Zurich, Switzerland) · 2008Article
- Predicting prognosis in gastroentero-pancreatic neuroendocrine tumors: an overview and the value of Ki-67 immunostaining.Endocrine pathology · 2008Review
- Comparison of Ki-67 equivalent antibodies.Journal of clinical pathology · 2002Article
- Comparison of vascularity and angiogenesis in primary invasive mammary carcinomas and in their respective axillary lymph node metastases.Clinical & experimental metastasis · 2000Article
- Co-expression of vascular endothelial growth factor (VEGF) and its receptors (flk-1 and flt-1) in hormone-induced mammary cancer in the Noble rat.British journal of cancer · 1999Article
- CD26 (Dipeptidyl Aminopeptidase IV) Expression in Normal and Diseased Human Thyroid Glands.Endocrine pathology · 1998Article
- Proliferative activity in human glioblastomas: evaluation of different Ki67 equivalent antibodies.Molecular pathology : MP · 1997Article
- Proliferative Activity in Pancreatic Endocrine Tumors: Association with Function, Metastases, and Survival.Endocrine pathology · 1997Article
- Ki-67 expression in early prostate cancer and associated pathological lesions.Journal of clinical pathology · 1996Article
- Proliferation indexes--a comparison between cutaneous basal and squamous cell carcinomas.Journal of clinical pathology · 1996Article
- Which proliferation markers for routine immunohistology?Journal of clinical pathology · 1996Article
- Effect of Ribonuclease A and Deoxyribonuclease I on Immunostaining of Ki-67 in Cultured Melanoma Cells.Pathology oncology research : POR · 1996Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo determine the best of five antibodies for immunohistochemical assessment of growth fraction in formalin fixed, paraffin wax embedded tissues.
methodsSections from 100 recent, and 17 ten year old or over wax embedded blocks of normal and malignant tissues were immunostained with monoclonal Ki67, polyclonal Ki67, PC10, MIB1, and JC1. The antibodies were evaluated for specificity of nuclear versus cytoplasmic staining, cleanliness of background, and compared with the expected pattern of staining in normal tissues, defined immunohistochemically by monoclonal Ki67 antibody in frozen tissues or by tritiated thymidine uptake.
resultsNo marker was ideal, but best results were obtained with MIB1 and polyclonal Ki67, followed by JC1, PC10, and monoclonal Ki67.
conclusionsFor routine use, MIB1 or polyclonal Ki67 are the best proliferation markers in conventional histological preparations. The other markers tested cannot be recommended.
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