ArticleJournal of assisted reproduction and genetics1994
Clinical experience with preimplantation diagnosis of sex by dual fluorescent in situ hybridization.
Article in Journal of assisted reproduction and genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Preimplantation genetic diagnosis for couples at high risk of Down syndrome pregnancy owing to parental translocation or mosaicism.Journal of medical genetics · 1999Trial
- The Effect of Assisted Reproductive Technologies on Human Sex Ratios.The Australian & New Zealand journal of obstetrics & gynaecology · 2025Article
- Assisted reproductive technology induces different secondary sex ratio: parental and embryonic impacts.Reproductive health · 2023Article
- The sex ratio of singleton and twin delivery offspring in assisted reproductive technology in China.Scientific reports · 2017Article
- Article
- Changes in sex ratio from fertilization to birth in assisted-reproductive-treatment cycles.Reproductive biology and endocrinology : RB&E · 2014Review
- Twenty-four chromosome FISH in human IVF embryos reveals patterns of post-zygotic chromosome segregation and nuclear organisation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Preimplantation genetic diagnosis: state of the art 2011.Human genetics · 2012Review
- Advances in embryo selection methods.F1000 biology reports · 2012Article
- In vitro fertilization (IVF): a review of 3 decades of clinical innovation and technological advancement.Therapeutics and clinical risk management · 2006Article
- The cytogenetics of preimplantation human development: insights provided by traditional and novel techniques.Chromosoma · 2005Review
- Protein nanotechnology - A powerful futuristic diagnostic technique.Indian journal of clinical biochemistry : IJCB · 2005Article
- Different probe combinations for assessment of postzygotic chromosomal imbalances in human embryos.Journal of assisted reproduction and genetics · 2002Article
- Improving the fixation method for preimplantation genetic diagnosis by fluorescent in situ hybridization.Journal of assisted reproduction and genetics · 1998Article
- Preimplantation diagnosis by FISH: the Rambam experience.Journal of assisted reproduction and genetics · 1998Article
- Pregnancies resulting from embryos biopsied for preimplantation diagnosis of genetic disease: biochemical and ultrasonic studies in the first trimester of pregnancy.Journal of assisted reproduction and genetics · 1996Article
- Preimplantation diagnosis of inherited disease by embryo biopsy: an update of the world figures.Journal of assisted reproduction and genetics · 1996Article
- Detection of chromosomal abnormalities in human preimplantation embryos using FISH.Journal of assisted reproduction and genetics · 1996Article
- Polar body diagnosis of common aneuploidies by FISH.Journal of assisted reproduction and genetics · 1996Article
- Review: preimplantation diagnosis of inherited disease.Journal of inherited metabolic disease · 1996Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeOur purpose was to assess the clinical application of dual fluorescent in situ hybridization (FISH) for the diagnosis of sex in the human preimplantation embryo.
resultsOver a 2-year period, 18 couples at risk of transmitting X-linked recessive disorders underwent preimplantation diagnosis of embryo sex by dual FISH with X and Y chromosome-specific DNA probes. A total of 27 in vitro fertilization (IVF) treatment cycles led to nine pregnancies; 7 reached the stage of clinical recognition, of which 2 spontaneously aborted. There were five live births, three singleton and two twin: none in disagreement with the diagnosed sex. The diagnosis was corroborated in 51 of the 74 nontransferred embryos. The efficiency of the procedure improved throughout the four treatment cycles. This was reflected in the increased proportion of double embryo transfers (from 50% in series 1 and 2 to 100% in series 3 and 4), with a consequent improvement in pregnancy rate (from 28 to 71% per embryo transfer). The excess of male embryos (male:female, 60:40 overall) and the high proportion of biopsied embryos with abnormal numbers of X and Y chromosome signals (14.5%) effectively reduced the number of normal female embryos available for transfer.
conclusionDual FISH is an efficient technique for determination of the sex of human preimplantation embryos and the additional ability to detect abnormal chromosome copy numbers, which is not possible via the polymerase chain reaction, (PCR), makes FISH the preferred technique.
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