ArticleThe Biochemical journal1995
Human proteasomes analysed with monoclonal antibodies.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 93 citations in OpenAlex.
- Proteasome dynamics in response to metabolic changes.Frontiers in cell and developmental biology · 2025Review
- Insulin-degrading enzyme regulates insulin-directed cellular autoimmunity in murine type 1 diabetes.Frontiers in immunology · 2024Article
- mTOR Modulates Methamphetamine-Induced Toxicity through Cell Clearing Systems.Oxidative medicine and cellular longevity · 2018Article
- The Autophagoproteasome a Novel Cell Clearing Organelle in Baseline and Stimulated Conditions.Frontiers in neuroanatomy · 2016Article
- The capture proteasome assay (CAPA) to evaluate subtype-specific proteasome inhibitors.Data in brief · 2015Article
- Protein-protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches.Cellular and molecular life sciences : CMLS · 2014Review
- Regulation of the proteasome by neuronal activity and calcium/calmodulin-dependent protein kinase II.The Journal of biological chemistry · 2009Article
- The ubiquitin-proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis.Parasitology research · 2009Article
- Affinity purification strategy to capture human endogenous proteasome complexes diversity and to identify proteasome-interacting proteins.Molecular & cellular proteomics : MCP · 2009Article
- Stable ubiquitination of human T-cell leukemia virus type 1 tax is required for proteasome binding.Journal of virology · 2004Article
- Plasticity in eucaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast.The EMBO journal · 2004Article
- A cytomegalovirus inhibitor of gamma interferon signaling controls immunoproteasome induction.Journal of virology · 2004Article
- Human T-cell leukemia virus type 1 Tax protein binds to assembled nuclear proteasomes and enhances their proteolytic activity.Journal of virology · 2001Article
- Degradation of transcription factor RFX5 during the inhibition of both constitutive and interferon gamma-inducible major histocompatibility complex class I expression in chlamydia-infected cells.The Journal of experimental medicine · 2000Article
- Hepatitis B virus X protein is both a substrate and a potential inhibitor of the proteasome complex.Journal of virology · 1999Article
- Intracellular localization of proteasomal degradation of a viral antigen.The Journal of cell biology · 1999Article
- Proteasome subunit zeta, a putative ribonuclease, is also found as a free monomer.Molecular biology reports · 1999Article
- Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes.The Biochemical journal · 1998Article
- Immunoproteasome assembly: cooperative incorporation of interferon gamma (IFN-gamma)-inducible subunits.The Journal of experimental medicine · 1998Article
- Us9, a stable lysine-less herpes simplex virus 1 protein, is ubiquitinated before packaging into virions and associates with proteasomes.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proteasome or multicatalytic endopeptidase from eukaryotic cells consists of at least 14 subunits that fall into two families, alpha and beta. Subunit-specific monoclonal antibodies against ten different subunits of human proteasomes have been produced, together with an antibody that reacts with a motif (prosbox 1), common to alpha-type subunits. Four of the subunit-specific antibodies were able to precipitate proteasomes. The subunit composition of HeLa-cell proteasomes precipitated with these four different antibodies were identical, as judged from two-dimensional electrophoresis. One of the four antibodies was used to obtain proteasomes from cell lines (HeLa, Daudi, IMR90 and BSC-1) and human tissues (placenta, kidney, and liver). Electrophoretic analysis of these proteasomes, combined with peptide mapping of some subunits, suggests that they all contain 14 types of subunits as their major constituents. However, one subunit was present in two isoelectric isoforms in all cells examined. Two other subunits occurred in two or three isoelectric isoforms in placenta, liver and kidney, but not in the cell cultures. Extracts of human cells (HeLa, IMR90, Daudi and erythrocytes) were analysed by non-denaturing electrophoresis and immunoblotting. All of the 11 subunits detected by antibodies were present in a pair of ATP-stabilized protein complexes, presumed to be the 26 S proteinase, and in a doublet of complexes which migrated more slowly than purified proteasomes. Besides being present in proteasomes, one subunit was also found to occur in the free state in cell extracts.
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Registered trials
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