ArticlePlant physiology1994
The regulation of gene expression in transformed maize aleurone and endosperm protoplasts. Analysis of promoter activity, intron enhancement, and mRNA untranslated regions on expression.
Article in Plant physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Transient expression of fluorescently tagged proteins in developing maize aleurone cells.MethodsX · 2021Article
- Rapid and efficient Agrobacterium-mediated transformation of sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker.Plant cell reports · 2016Article
- Article
- Agrobacterium tumefaciens-mediated transformation of maize endosperm as a tool to study endosperm cell biology.Plant physiology · 2010Article
- Design of gene constructs for transgenic maize.Methods in molecular biology (Clifton, N.J.) · 2009Review
- Gene expression enhancement mediated by the 5' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants.Molecular genetics and genomics : MGG · 2008Article
- Optimization of Acidothermus cellulolyticus endoglucanase (E1) production in transgenic tobacco plants by transcriptional, post-transcription and post-translational modification.Transgenic research · 2005Article
- Untranslated regions from C4 amaranth AhRbcS1 mRNAs confer translational enhancement and preferential bundle sheath cell expression in transgenic C4 Flaveria bidentis.Plant physiology · 2004Article
- ARC-1, a sequence element complementary to an internal 18S rRNA segment, enhances translation efficiency in plants when present in the leader or intercistronic region of mRNAs.Nucleic acids research · 2004Article
- Analysis of regulatory elements of the promoter and the 3' untranslated region of the maize Hrgp gene coding for a cell wall protein.Plant cell reports · 2003Article
- Requirements for intron-mediated enhancement of gene expression in Arabidopsis.RNA (New York, N.Y.) · 2002Article
- Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-rich motif increases expression without affecting splicing.Plant physiology · 2002Article
- Introns and their positions affect the translational activity of mRNA in plant cells.EMBO reports · 2001Article
- Sucrose export defective1 encodes a novel protein implicated in chloroplast-to-nucleus signaling.The Plant cell · 2001Article
- Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis.Plant molecular biology · 2001Article
- The Caenorhabditis elegans gene unc-25 encodes glutamic acid decarboxylase and is required for synaptic transmission but not synaptic development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Both 5' and 3' sequences of maize adh1 mRNA are required for enhanced translation under low-oxygen conditions.Plant physiology · 1996Article
- Optimizing expression of transgenes with an emphasis on post-transcriptional events.Plant molecular biology · 1996Review
- Translation in plants--rules and exceptions.Plant molecular biology · 1996Review
- Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery.Plant molecular biology · 1996Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression in the aleurone and endosperm is highly regulated during both seed development and germination. Studies of alpha-amylase expression in the aleurone of barley (Hordeum vulgare) have generated the current paradigm for hormonal control of gene expression in germinating cereal grain. Gene expression studies in both the aleurone and endosperm tissues of maize (Zea mays) seed have been hampered because of a lack of an efficient transformation system. We report here the rapid isolation of protoplasts from maize aleurone and endosperm tissue, their transformation using polyethylene glycol or electroporation, and the regulation of gene expression in these cells. Adh1 promoter activity was reduced relative to the 35S promoter in aleurone and endosperm protoplasts compared to Black Mexican Sweet suspension cells in which it was nearly as strong as the 35S promoter. Intron-mediated stimulation of expression was substantially higher in transformed aleurone or endosperm protoplasts than in cell-suspension culture protoplasts, and the data suggest that the effect of an intron may be affected by cell type. To examine cytoplasmic regulation, the 5' and 3' untranslated regions from a barley alpha-amylase were fused to the firefly luciferase-coding region, and their effect on translation and mRNA stability was examined following the delivery of in vitro synthesized mRNA to aleurone and endosperm protoplasts. The alpha-amylase untranslated regions regulated translational efficiency in a tissue-specific manner, increasing translation in aleurone or endosperm protoplasts but not in maize or carrot cell-suspension protoplasts, in animal cells, or in in vitro translation lysates.
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