ArticleMolecular and cellular biology1995
Transcriptional regulation of the mouse alpha A-crystallin gene: activation dependent on a cyclic AMP-responsive element (DE1/CRE) and a Pax-6-binding site.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 129 citations in OpenAlex.
- Article
- Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers.Experimental eye research · 2019Article
- Signaling and Gene Regulatory Networks in Mammalian Lens Development.Trends in genetics : TIG · 2017Review
- Klotho regulates retinal pigment epithelial functions and protects against oxidative stress.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Pax6 interactions with chromatin and identification of its novel direct target genes in lens and forebrain.PloS one · 2013Article
- Patterns of gene expression in microarrays and expressed sequence tags from normal and cataractous lenses.Human genomics · 2012Article
- Retinoid signaling in inner ear development: A "Goldilocks" phenomenon.American journal of medical genetics. Part A · 2010Review
- Oocyte-type linker histone B4 is required for transdifferentiation of somatic cells in vivo.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Pax6 is essential for lens fiber cell differentiation.Development (Cambridge, England) · 2009Article
- The molecular basis of defective lens development in the Iberian mole.BMC biology · 2008Article
- Notch signaling regulates growth and differentiation in the mammalian lens.Developmental biology · 2008Article
- Genetic and epigenetic mechanisms of gene regulation during lens development.Progress in retinal and eye research · 2007Review
- Distinct embryonic expression and localization of CBP and p300 histone acetyltransferases at the mouse alphaA-crystallin locus in lens.Journal of molecular biology · 2007Article
- The MH1 domain of Smad3 interacts with Pax6 and represses autoregulation of the Pax6 P1 promoter.Nucleic acids research · 2007Article
- Regulation of alphaA-crystallin via Pax6, c-Maf, CREB and a broad domain of lens-specific chromatin.The EMBO journal · 2006Article
- Crystallization and preliminary X-ray analysis of the Pax6 paired domain bound to the Pax6 gene enhancer.Acta crystallographica. Section F, Structural biology and crystallization communications · 2005Article
- Role of Fabp7, a downstream gene of Pax6, in the maintenance of neuroepithelial cells during early embryonic development of the rat cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Tissue-specific regulation of the mouse alphaA-crystallin gene in lens via recruitment of Pax6 and c-Maf to its promoter.Journal of molecular biology · 2005Article
- Functional properties of natural human PAX6 and PAX6(5a) mutants.Investigative ophthalmology & visual science · 2004Article
- Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens.The International journal of developmental biology · 2004Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two cis-acting promoter elements (-108 to -100 and -49 to -33) of the mouse alpha A-crystallin gene, which is highly expressed in the ocular lens, were studied. Here we show that DE1 (-108 to -100; 5'TGACGGTG3'), which resembles the consensus cyclic AMP (cAMP)-responsive element sequence (CRE; 5'TGACGT[A/C][A/G]3'), behaves like a functional CRE site. Transfection experiments and electrophoretic mobility shift assays (EMSAs) using site-specific mutations correlated a loss of function with deviations from the CRE consensus sequence. Results of EMSAs in the presence of antisera against CREB, delta CREB, and CREM were consistent with the binding of CREB-like proteins to the DE1 sequence. Stimulation of alpha A-crystallin promoter activity via 8-bromo-cAMP, forskolin, or human T-cell leukemia virus type I Tax1 in transfections and reduction of activity of this site in cell-free transcription tests by competition with the somatostatin CRE supported the idea that DE1 is a functional CRE. Finally, Pax-6, a member of the paired-box family of transcription factors, activated the mouse alpha A-crystallin promoter in cotransfected COP-8 fibroblasts and bound to the -59 to -29 promoter sequence in EMSAs. These data provide evidence for a synergistic role of Pax-6 and CREB-like proteins for high expression of the mouse alpha A-crystallin gene in the lens.
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