ArticleThe Journal of cell biology1995
Drosophila development requires spectrin network formation.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Generation and maintenance of apical rib-like actin fibers in epithelial support cells of the Drosophila eye.Development (Cambridge, England) · 2026Article
- αII-spectrin and βII-spectrin do not affect TGFβ1-induced myofibroblast differentiation.Cell and tissue research · 2018Article
- Spectrin tetramer formation is not required for viable development in Drosophila.The Journal of biological chemistry · 2015Article
- Genetic studies of spectrin in the larval fat body of Drosophila melanogaster: evidence for a novel lipid uptake apparatus.Genetics · 2013Article
- Orbit/CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males.PloS one · 2013Article
- Tropomodulins: pointed-end capping proteins that regulate actin filament architecture in diverse cell types.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Transgene rescue identifies an essential function for Drosophila beta spectrin in the nervous system and a selective requirement for ankyrin-2-binding activity.Molecular biology of the cell · 2010Article
- Tropomodulin1 is required for membrane skeleton organization and hexagonal geometry of fiber cells in the mouse lens.The Journal of cell biology · 2009Article
- Sequential degradation of alphaII and betaII spectrin by calpain in glutamate or maitotoxin-stimulated cells.Biochemistry · 2007Article
- Spectrin functions upstream of ankyrin in a spectrin cytoskeleton assembly pathway.The Journal of cell biology · 2006Article
- Mammalian alpha I-spectrin is a neofunctionalized polypeptide adapted to small highly deformable erythrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Spectrin alpha II and beta II isoforms interact with high affinity at the tetramerization site.The Biochemical journal · 2003Article
- betaIV spectrin, a new spectrin localized at axon initial segments and nodes of ranvier in the central and peripheral nervous system.The Journal of cell biology · 2000Article
- Apical spectrin is essential for epithelial morphogenesis but not apicobasal polarity in Drosophila.The Journal of cell biology · 1999Article
- Spectrin self-association site: characterization and study of beta-spectrin mutations associated with hereditary elliptocytosis.The Biochemical journal · 1998Article
- Neuroglian-mediated cell adhesion induces assembly of the membrane skeleton at cell contact sites.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The head-end associations of spectrin give rise to tetramers and make it possible for the molecule to form networks. We analyzed the head-end associations of Drosophila spectrin in vitro and in vivo. Immunoprecipitation assays using protein fragments synthesized in vitro from recombinant DNA showed that interchain binding at the head end was mediated by segment 0-1 of alpha-spectrin and segment 18 of beta-spectrin. Point mutations equivalent to erythroid spectrin mutations that are responsible for human hemolytic anemias diminished Drosophila spectrin head-end interchain binding in vitro. To test the in vivo consequence of deficient head-end interchain binding, we introduced constructs expressing head-end interchain binding mutant alpha-spectrin into the Drosophila genome and tested for rescue of an alpha-spectrin null mutation. An alpha-spectrin minigene lacking the codons for head-end interchain binding failed to rescue the lethality of the null mutant, whereas a minigene with a point mutation in these codons overcame the lethality of the null mutant in a temperature-dependent manner. The rescued flies were viable and fertile at 25 degrees C, but they became sterile because of defects in oogenesis when shifted to 29 degrees C. At 29 degrees C, egg chamber tissue disruption and cell shape changes were evident, even though the mutant spectrin remained stably associated with cell membranes. Our results show that spectrin's capacity to form a network is a crucial aspect of its function in nonerythroid cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.