ArticleThe Journal of cell biology1995
Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 198 citations in OpenAlex.
- A cellular atlas of calcineurin signaling.Biochimica et biophysica acta. Molecular cell research · 2023Review
- The Nuclear Envelope as a Regulator of Immune Cell Function.Frontiers in immunology · 2022Review
- Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling.Molecular cell · 2020Article
- The nucleoporin gp210/Nup210 controls muscle differentiation by regulating nuclear envelope/ER homeostasis.The Journal of cell biology · 2015Article
- Nesprin-2 mediated nuclear trafficking and its clinical implications.Nucleus (Austin, Tex.) · 2015Article
- Bcl-2 family in inter-organelle modulation of calcium signaling; roles in bioenergetics and cell survival.Journal of bioenergetics and biomembranes · 2014Review
- Activation of intracellular calcium by multiple Wnt ligands and translocation of β-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/β-catenin pathways.The Journal of biological chemistry · 2013Article
- Intracellular organelles in the saga of Ca2+ homeostasis: different molecules for different purposes?Cellular and molecular life sciences : CMLS · 2012Review
- Calcium signaling in synapse-to-nucleus communication.Cold Spring Harbor perspectives in biology · 2011Review
- Calcium regulation of nucleocytoplasmic transport.Protein & cell · 2011Review
- Dynamic access of the glucocorticoid receptor to response elements in chromatin.The international journal of biochemistry & cell biology · 2009Review
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- Calcium signals can freely cross the nuclear envelope in hippocampal neurons: somatic calcium increases generate nuclear calcium transients.BMC neuroscience · 2007Article
- Hormonal regulation of nuclear permeability.The Journal of biological chemistry · 2007Article
- Route of glucocorticoid-induced macromolecules across the nuclear envelope as viewed by atomic force microscopy.Pflugers Archiv : European journal of physiology · 2006Review
- Chromatin dynamics and the evolution of alternate promoter states.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2006Review
- Host cell nuclear proteins are recruited to cytoplasmic vaccinia virus replication complexes.Journal of virology · 2005Article
- Ca2+ signaling, intracellular pH and cell volume in cell proliferation.The Journal of membrane biology · 2005Review
- Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1.Molecular biology of the cell · 2005Article
- Live imaging of glucose homeostasis in nuclei of COS-7 cells.Journal of fluorescence · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear pore complexes provide channels for molecular transport across the nuclear envelope. Translocation of most proteins and RNAs through the pore complex is mediated by signal- and ATP-dependent mechanisms, while transport of small molecules is accomplished by passive diffusion. We report here that depletion of calcium from the lumen of the endoplasmic reticulum and nuclear envelope with ionophores or the calcium pump inhibitor thapsigargin rapidly and potently inhibits signal mediated transport of proteins into the nucleus. Lumenal calcium depletion also inhibits passive diffusion through the pore complex. Signal-mediated protein import and passive diffusion are rapidly restored when the drugs depleting lumenal calcium are removed and cells are incubated at 37 degrees C in calcium-containing medium. These results indicate that loss of calcium from the lumen of the endoplasmic reticulum and nuclear envelope reversibly affects properties of pore complex components located on the nuclear/cytoplasmic membrane surfaces, and they provide direct functional evidence for conformational flexibility of the pore complex. These methods will be useful for achieving reversible inhibition of nucleocytoplasmic trafficking for in vivo functional studies, and for studying the structure of the passive diffusion channel(s) of the pore complex.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.