ArticleThe Journal of cell biology1995
Type X collagen gene expression in mouse chondrocytes immortalized by a temperature-sensitive simian virus 40 large tumor antigen.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 75 citations in OpenAlex.
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- Juvenile idiopathic arthritis fibroblast-like synoviocytes influence chondrocytes to alter BMP antagonist expression demonstrating an interaction between the two prominent cell types involved in endochondral bone formation.Pediatric rheumatology online journal · 2020Article
- TAp63γ influences mouse cartilage development.Aging · 2020Article
- Expression of RMRP RNA is regulated in chondrocyte hypertrophy and determines chondrogenic differentiation.Scientific reports · 2017Article
- In vitro functional characterization of prostaglandin-endoperoxide synthase 2 during chondrocyte hypertrophic differentiation.Oncotarget · 2016Article
- Runx2-interacting genes identified by yeast two-hybrid screening of libraries generated from hypertrophic chondrocytes.American journal of translational research · 2016Article
- Collagen-mimetic peptide-modifiable hydrogels for articular cartilage regeneration.Biomaterials · 2015Article
- Identification and characterization of the novel Col10a1 regulatory mechanism during chondrocyte hypertrophic differentiation.Cell death & disease · 2014Article
- microRNA-495 inhibits chondrogenic differentiation in human mesenchymal stem cells by targeting Sox9.Stem cells and development · 2014Article
- Matrilin-2 is proteolytically cleaved by ADAMTS-4 and ADAMTS-5.Molecules (Basel, Switzerland) · 2014Article
- Phenotypic plasticity in normal breast derived epithelial cells.BMC cell biology · 2014Article
- Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.Zebrafish · 2013Article
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- Mouse p63 variants and chondrogenesis.International journal of clinical and experimental pathology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mouse endochondral chondrocytes were immortalized with a temperature-sensitive simian virus 40 large tumor antigen. Several clonal isolates as well as pools of immortalized cells were characterized. In monolayer cultures at the temperature permissive for the activity of the large tumor antigen (32 degrees C), the cells grew continuously with a doubling time of approximately 2 d, whereas they stopped growing at nonpermissive temperatures (37 degrees C-39 degrees C). The cells from all pools and from most clones expressed the genes for several markers of hypertrophic chondrocytes, such as type X collagen, matrix Gla protein, and osteopontin, but had lost expression of type II collagen mRNA and failed to be stained by alcian blue which detects cartilage-specific proteoglycans. The cells also contained mRNAs for type I collagen and bone Gla protein, consistent with acquisition of osteoblastic-like properties. Higher levels of mRNAs for type X collagen, bone Gla protein, and osteopontin were found at nonpermissive temperatures, suggesting that the expression of these genes was upregulated upon growth arrest, as is the case in vivo during chondrocyte hypertrophy. Cells also retained their ability to respond to retinoic acid, as indicated by retinoic acid dose-dependent and time-dependent increases in type X collagen mRNA levels. These cell lines, the first to express characteristic features of hypertrophic chondrocytes, should be very useful to study the regulation of the type X collagen gene and other genes activated during the last stages of chondrocyte differentiation.
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