ArticleThe Journal of cell biology1995
Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 217 citations in OpenAlex.
- Leaving no-one behind: how CENP-E facilitates chromosome alignment.Essays in biochemistry · 2020Review
- Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells.Cell death discovery · 2020Article
- α-Fodrin is required for the organization of functional microtubules during mitosis.Cell cycle (Georgetown, Tex.) · 2019Article
- A tension-independent mechanism reduces Aurora B-mediated phosphorylation upon microtubule capture by CENP-E at the kinetochore.Cell cycle (Georgetown, Tex.) · 2019Article
- Phosphatases in Mitosis: Roles and Regulation.Biomolecules · 2019Review
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Review
- Mitosis.Cold Spring Harbor perspectives in biology · 2016Review
- Cenp-meta is required for sustained spindle checkpoint.Biology open · 2014Article
- Kinetochore kinesin CENP-E is a processive bi-directional tracker of dynamic microtubule tips.Nature cell biology · 2013Article
- New insights into the mechanism for chromosome alignment in metaphase.International review of cell and molecular biology · 2013Review
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Mitosis futures: the past is prologue.Molecular biology of the cell · 2011Article
- Tubulin depolymerization may be an ancient biological motor.Journal of cell science · 2010Review
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Contrasting models for kinetochore microtubule attachment in mammalian cells.Cellular and molecular life sciences : CMLS · 2010Review
- Mitotic force generators and chromosome segregation.Cellular and molecular life sciences : CMLS · 2010Review
- Mechanisms of chromosome behaviour during mitosis.Nature reviews. Molecular cell biology · 2010Review
- Reduced expression of cenp-e in human hepatocellular carcinoma.Journal of experimental & clinical cancer research : CR · 2009Article
- Characterization of novel diaryl oxazole-based compounds as potential agents to treat pancreatic cancer.The Journal of pharmacology and experimental therapeutics · 2009Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Chromosomes can move with the ends of depolymerizing microtubules (MTs) in vitro, even in the absence of nucleotide triphosphates (Coue, M., V. A. Lombillo, and J. R. McIntosh. 1991. J. Cell Biol. 112:1165-1175.) Here, we describe an immunological investigation of the proteins important for this form of motility. Affinity-purified polyclonal antibodies to kinesin exert a severe inhibitory effect on depolymerization-dependent chromosome motion. These antibodies predominantly recognize a polypeptide of M(r) approximately 250 kD on immunoblots of CHO chromosomes and stain kinetochores as well as some vesicles that are in the chromosome preparation. Antibodies to CENP-E, a kinetochore-associated kinesin-like protein, also recognize a 250-kD electrophoretic component, but they stain only the kinetochroe region of isolated chromosomes. Polyclonal antibodies that recognize specific domains of the CENP-E polypeptide affect MT disassembly-dependent chromosome motion in different ways; antibodies to the head or tail portions slow motility threefold, while those raised against the neck region stop motion completely. Analogous antibodies that block conventional, ATP-dependent motility of cytoplasmic dynein (Vaisberg, G., M. P. Koonce, and J. R. McIntosh. 1993. J. Cell Biol. 123:849-858) have no effect on disassembly-dependent chromosome motion, even though they bind to kinetochores. These observations suggest that CENP-E helps couple chromosomes to depolymerizing MTs. A similar coupling activity may allow spindle MTs to remain kinetochore-bound while their lengths change during both prometaphase and anaphase A.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.