ArticleExperimental neurology1994
Morphine inhibits Purkinje cell survival and dendritic differentiation in organotypic cultures of the mouse cerebellum.
Article in Experimental neurology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Associations between opioid exposure and brain size among extremely preterm infants.Journal of perinatology : official journal of the California Perinatal Association · 2026Article
- Early-Life Clinical Factors Shape Cerebellar Structure in Preterm-Born Children.Annals of the Child Neurology Society · 2026Article
- Impact of Intrauterine Insults on Fetal and Postnatal Cerebellar Development in Humans and Rodents.Cells · 2024Review
- Brain health in preterm infants: importance of early-life pain and analgesia exposure.Pediatric research · 2024Review
- Body size and brain volumetry in the rat following prolonged morphine administration in infancy and adulthood.Frontiers in pain research (Lausanne, Switzerland) · 2023Article
- Purkinje cell-specific deletion of CREB worsens alcohol-induced cerebellar neuronal losses and motor deficits.Alcohol (Fayetteville, N.Y.) · 2022Article
- Association between morphine exposure and impaired brain development on term-equivalent age brain magnetic resonance imaging in very preterm infants.Scientific reports · 2022Article
- Fentanyl Exposure in Preterm Infants: Five-Year Neurodevelopmental and Socioemotional Assessment.Frontiers in pain research (Lausanne, Switzerland) · 2022Article
- Long-term impact of systematic pain and sedation management on cognitive, motor, and behavioral outcomes of extremely preterm infants at preschool age.Pediatric research · 2021Observational
- Brain growth in the NICU: critical periods of tissue-specific expansion.Pediatric research · 2018Article
- Short-term incubation of gabapentin or pregabalin does not affect chemically induced injury in neuronal cell models in vitro.Journal of pain research · 2018Article
- Fentanyl Induces Cerebellar Internal Granular Cell Layer Apoptosis in Healthy Newborn Pigs.Frontiers in neurology · 2018Article
- Opiate Drugs with Abuse Liability Hijack the Endogenous Opioid System to Disrupt Neuronal and Glial Maturation in the Central Nervous System.Frontiers in pediatrics · 2017Review
- Smaller Cerebellar Growth and Poorer Neurodevelopmental Outcomes in Very Preterm Infants Exposed to Neonatal Morphine.The Journal of pediatrics · 2016Article
- Brain Injury and Development in Preterm Infants Exposed to Fentanyl.The Annals of pharmacotherapy · 2015Article
- Prenatal morphine exposure reduces pyramidal neurons in CA1, CA2 and CA3 subfields of mice hippocampus.Iranian journal of basic medical sciences · 2014Article
- Opiate drug use and the pathophysiology of neuroAIDS.Current HIV research · 2012Review
- Purkinje cells loss in off spring due to maternal morphine sulfate exposure: a morphometric study.Anatomy & cell biology · 2012Article
- Interactive comorbidity between opioid drug abuse and HIV-1 Tat: chronic exposure augments spine loss and sublethal dendritic pathology in striatal neurons.The American journal of pathology · 2010Article
- Ionic storm in hypoxic/ischemic stress: can opioid receptors subside it?Progress in neurobiology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The effects of morphine on the morphogenesis and survival of calbindin-D28k-immunoreactive Purkinje cells were studied in organotypic explant cultures isolated from 1- or 7-day-old mouse cerebella. To reduce experimental variability, bilaterally matched pairs of organotypic cultures were used to compare the effects of opiate drug treatment. One explant within each pair was untreated, while the remaining explant was continuously treated for 7 to 10 days with morphine, morphine plus naloxone, or naloxone alone. In explants derived from 1-day-old mice, morphine treatment significantly reduced Purkinje cell dendritic length compared to symmetrically matched untreated control explants. The concentration of morphine estimated to cause a half-maximal reduction (EC50) in dendritic length was 4.9 x 10(-8) M. At higher concentrations (EC50 = 3.6 x 10(-6) M), morphine also significantly decreased the number of Purkinje cells in explants from 1-day-old mice compared to untreated explants. Electron microscopy identified increased numbers of degenerating Purkinje cells in explants derived from 1-day-old mice. This showed that high concentrations (10(-5) M) of morphine reduced Purkinje cell numbers by decreasing their rate of survival. In explants derived from 7-day-old mice, morphine (10(-5) M) neither affected Purkinje cell dendritic length nor cell numbers compared to symmetrically matched untreated (control) explants. Collectively, these findings suggest that morphine per se, through a direct action on the cerebellum, can affect Purkinje cell differentiation and survival. The results additionally suggest that there is a critical period during development when Purkinje cells are especially vulnerable to the effects of morphine.
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Registered trials
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