Evidence map›Paper›PMID 7819123›Full record

ArticleInternational journal of legal medicine1994

The validation of short tandem repeat (STR) loci for use in forensic casework.

J E Lygo, P E Johnson, D J Holdaway, S Woodroffe, J P Whitaker, T M Clayton, C P Kimpton, P Gill

Abstract read
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In one paragraph

Article in International journal of legal medicine, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
7.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 223 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Cancer and forensic microsatellites.Forensic science, medicine, and pathology · 2008
    Review
  9. DNA reviews: the national DNA database of the United Kingdom.Forensic science, medicine, and pathology · 2007
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Evaluation of new primers for CSF1PO.International journal of legal medicine · 1997
    Article
  20. A Dutch population study of the STR loci D21S11 and HUMFIBRA.International journal of legal medicine · 1997
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

J E LygoForensic Science Service, Reading, Berkshire, UK.
P E Johnson
D J Holdaway
S Woodroffe
J P Whitaker
T M Clayton
C P Kimpton
P Gill
Atomic Weapons Establishment · GBThe Priory Hospital · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A quadruplex reaction has been developed which amplifies the short tandem repeat (STR) loci HUM-VWA31/A, HUMTHO1, HUMF13A1 and HUMFES/FPS. Detection of the PCR products employs denaturing polyacrylamide gels coupled with fluorescent-based technology. This system has been evaluated for use in routine forensic casework and has been shown to be both robust and reproducible. The quadruplex reaction is as sensitive as the commercially available HLA DQ alpha Amplitype typing system and can be used on both degraded and aged material. The problems of environmental contamination have been shown to be limited provided strict procedural practices are followed-i.e. physical separation of sample extraction and amplified products; the use of dedicated equipment such as pipettes; the separation of amplification preparation area. The ability of the system to detect mixtures and the successful analysis of case stains has shown that this system is well suited as a tool for forensic investigation.

Indexed as

Forensic MedicineAllelesAnimalsBase SequenceBirdsChromosome MappingChromosomes, Human, Pair 17DNADNA, SatelliteEnvironmentGene AmplificationHLA-DQ alpha-ChainsHLA-DQ AntigensHumansMammalsPolymerase Chain ReactionDNADNA, SatelliteHLA-DQA1 antigenHLA-DQ alpha-ChainsHLA-DQ Antigens

Identifiers

PMID7819123
OpenAlexW2043788447

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.