ArticleThe Biochemical journal1994
The gene family of EF-hand calcium-binding proteins from the flagellum of Trypanosoma brucei.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Review
- Flagellar membrane proteins in kinetoplastid parasites.IUBMB life · 2015Review
- Calcium signaling in trypanosomatid parasites.Cell calcium · 2015Review
- (1)H, (15)N, and (13)C chemical shift assignments of the calflagin Tb24 flagellar calcium binding protein of Trypanosoma brucei.Biomolecular NMR assignments · 2013Article
- Bloodstream form pre-adaptation to the tsetse fly in Trypanosoma brucei.Frontiers in cellular and infection microbiology · 2013Review
- NMR structure of the calflagin Tb24 flagellar calcium binding protein of Trypanosoma brucei.Protein science : a publication of the Protein Society · 2012Article
- Independent analysis of the flagellum surface and matrix proteomes provides insight into flagellum signaling in mammalian-infectious Trypanosoma brucei.Molecular & cellular proteomics : MCP · 2011Article
- Differential protein expression throughout the life cycle of Trypanosoma congolense, a major parasite of cattle in Africa.Molecular and biochemical parasitology · 2011Article
- Identification, molecular and functional characterization of calmodulin gene of Phytomonas serpens 15T that shares high similarity with its pathogenic counterparts Trypanosoma cruzi.The protein journal · 2011Article
- Composition and sensory function of the trypanosome flagellar membrane.Current opinion in microbiology · 2010Review
- Calflagin inhibition prolongs host survival and suppresses parasitemia in Trypanosoma brucei infection.Eukaryotic cell · 2010Article
- The paraflagellar rod of kinetoplastid parasites: from structure to components and function.International journal for parasitology · 2010Review
- Identification of a palmitoyl acyltransferase required for protein sorting to the flagellar membrane.Journal of cell science · 2009Article
- The Trypanosoma brucei flagellum: moving parasites in new directions.Annual review of microbiology · 2009Review
- Flagellar membrane trafficking in kinetoplastids.Parasitology research · 2007Review
- Use of full-length recombinant calflagin and its c fragment for improvement of diagnosis of Trypanosoma cruzi infection.Journal of clinical microbiology · 2005Article
- Biology and mechanism of trypanosome cell motility.Eukaryotic cell · 2003Review
- An RNA polymerase II promoter in the hsp70 locus of Trypanosoma brucei.Molecular and cellular biology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The flagellum of Trypanosoma brucei contains calmodulin, and a separate family of antigenically related EF-hand calcium-binding proteins which we call calflagins. The following study evaluates the structure and genomic organization of the calflagin family. Genomic Southern blots indicated that multiple copies of calflagin genes occurred in T. brucei, and that all of these copies were contained in a single 23 kb XhoI-XhoI fragment on chromosomes 15 and 16 mRNAs of 1.2 and 1.6 kb were identified in bloodstream and procyclic life-cycle stages. Genomic fragments of 2.5 and 1.7 kb were cloned that encoded calflagin sequences. The calflagin genes were arranged tandemly along the genomic fragments. Three new members of the calflagin family were sequenced from a cDNA clone and the two genomic clones. Two unrelated families of 3' flanking sequences were downstream from the calflagin genes. An open reading frame that was unrelated to any calflagin sequence was at the 5' end of the 2.5 kb genomic fragment. The deduced amino acid sequences of the genomic clones (called Tb-24 and Tb-1.7g) were similar to the previously described Tb-17. Each encoded an approximately 24 kDa protein which contained three EF-hand calcium-binding motifs and one degenerate EF-hand motif. The cDNA encoded a protein (called Tb-44A) which was approximately twice as large as the other calflagins. The large size resulted from a nearly direct repeat of 186 amino acids. In general, variability among the T. brucei calflagins was greater than observed for related proteins from Trypanosoma cruzi. We demonstrate that this variability resulted from amino acid substitutions at the N-terminus, C-terminal extensions, and duplication of internal segments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.