ArticleJournal of clinical microbiology1994
Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 108 citations in OpenAlex.
- Outbreak of Respiratory Disease Due to Bovine Respiratory Syncytial Virus with Concomitant Infections byAnimals : an open access journal from MDPI · 2025Article
- Pathological and molecular findings associated with BRSV in cattle and buffaloes with pulmonary disease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Development of a Novel, Non-Invasive Saliva Sampling Method for the Detection of Bovine Respiratory Viruses.Veterinary sciences · 2025Article
- Assessment of Risk Factors Related to Environmental Factors and Herd Management for Bovine Respiratory Syncytial Virus and Bovine Parainfluenza Virus-3 Infections Frequently Observed in Beef and Dairy Cattle.Veterinary medicine and science · 2025Article
- A surrogate in vitro experimental model for off-label drug repurposing: inhibitory effect of montelukast on bovine respiratory syncytial virus replication.Virology journal · 2025Article
- Respiratory illness in young and adult cattle caused by bovine viral diarrhea virus subgenotype 2b in singular and mixed bacterial infection in a BVDV-vaccinated dairy herd.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Establishment of a Real-Time Reverse Transcription Recombinase-Aided Isothermal Amplification (qRT-RAA) Assay for the Rapid Detection of Bovine Respiratory Syncytial Virus.Veterinary sciences · 2024Article
- Microbiological Profile of the Upper and Lower Respiratory Tract of Suckling and Weaned Dairy Calves with Acute Respiratory Disease.Veterinary sciences · 2024Article
- The Role ofPathogens (Basel, Switzerland) · 2024Article
- Neglected bacterial infections associated to bovine respiratory disease in lactating cows from high-yielding dairy cattle herds.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Bovine respiratory syncytial virus infection in feedlot cattle cases in Argentina.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Histophilus somni disease conditions with simultaneous infections by ovine gammaherpesvirus 2 in cattle herds from Southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle.Scientific reports · 2023Article
- Possible Association of Bovine Gammaherpesvirus 6 with Pulmonary Disease in a Cow.Animals : an open access journal from MDPI · 2023Article
- Cattle influenza D virus in Brazil is divergent from established lineages.Archives of virology · 2022Article
- Ovine gammaherpesvirus 2 infections in cattle without typical manifestations of sheep-associated malignant catarrhal fever and concomitantly infected with bovine coronavirus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Bovine Coronavirus Co-infection and Molecular Characterization in Dairy Calves With or Without Clinical Respiratory Disease.Frontiers in veterinary science · 2022Article
- Article
- Molecular characterization and comparison of diagnostic methods for bovine respiratory viruses (BPIV-3, BRSV, BVDV, and BoHV-1) in field samples in northwestern Turkey.Tropical animal health and production · 2021Article
- Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus.The veterinary quarterly · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two nested PCR assays were developed for the detection of bovine respiratory syncytial virus (BRSV). Primers were selected from the gene encoding the F fusion protein (PCR-F) and the gene encoding the G attachment protein (PCR-G). Biotinylated oligonucleotide probes, termed F and G, were selected for the hybridization of the respective PCR products. The sensitivities of the PCR-F and PCR-G assays were similar, both detecting 0.1 tissue culture infective dose of the virus. The PCR-F assay amplified all bovine strains and one human strain (RS32) tested. No cross-reactions were observed with nine heterologous respiratory viruses. PCR-F products of bovine and human RSV strains were discriminated by using endonuclease restriction enzyme ScaI, which specifically cleaved, products of BRSV. Oligonucleotide probe F was also specific for products of BRSV. The PCR-G assay detected all bovine strains and none of the human strains tested. A faint electrophoretic band was also observed with products of Sendai virus. However, probe G did not hybridize with this product, only with products of BRSV. Nasal swabs collected from cattle with no symptoms and cattle in the acute stage of respiratory disease were analyzed for BRSV by the immunofluorescence (IF) method and by the PCR-F and PCR-G assays. The virus was detected by the PCR assays in 31 of 35 (89%) samples tested. Only 23 samples (66%) were positive by the IF method, and these samples were also positive by both the PCR-F and PCR-G assays. The 31 samples detected as positive by PCR originated from cattle presenting clinical signs of acute respiratory disease; the four PCR-negative samples originated from clinically asymptomatic neighboring cattle. All sampled animals subsequently seroconverted and became reactive to BRSV. Thus, the detection of BRSV by PCR correlated with clinical observations and was considerably more sensitive (66 versus 89%) than IF. These results indicate that both nested PCR assays provide rapid and sensitive means for the detection of BRSV infection in cattle. Considering its higher specificity, the PCR-F assay can be recommended as the method of choice in the analysis of clinical specimens of BRSV.
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