ArticleJournal of clinical microbiology1994
Rendering of mycobacteria safe for molecular diagnostic studies and development of a lysis method for strand displacement amplification and PCR.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Discrimination betweenOne health (Amsterdam, Netherlands) · 2026Article
- The Biosafety Research Road Map: The Search for Evidence to Support Practices in the Laboratory-Zoonotic Avian Influenza andApplied biosafety : journal of the American Biological Safety Association · 2023Review
- An influence of dew point temperature on the occurrence of Mycobacterium tuberculosis disease in Chennai, India.Scientific reports · 2022Article
- Rapid Identification ofFrontiers in microbiology · 2022Article
- Biosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis.Microbiology spectrum · 2021Article
- DNA Thermo-Protection Facilitates Whole-Genome Sequencing of Mycobacteria Direct from Clinical Samples.Journal of clinical microbiology · 2020Article
- Heat Inactivation Renders Sputum Safe and PreservesJournal of clinical microbiology · 2019Article
- Genomic characterization ofMicrobial genomics · 2016Article
- Clonal Diversification and Changes in Lipid Traits and Colony Morphology in Mycobacterium abscessus Clinical Isolates.Journal of clinical microbiology · 2015Article
- Mycobacterium tuberculosis lineage 7 strains are associated with prolonged patient delay in seeking treatment for pulmonary tuberculosis in Amhara Region, Ethiopia.Journal of clinical microbiology · 2015Article
- Semi-automated, occupationally safe immunofluorescence microtip sensor for rapid detection of Mycobacterium cells in sputum.PloS one · 2014Article
- Bovine tuberculosis at a cattle-small ruminant-human interface in Meskan, Gurage region, Central Ethiopia.BMC infectious diseases · 2011Article
- Inactivation of cultured Mycobacterium tuberculosis organisms prior to DNA extraction.Journal of clinical microbiology · 2006Article
- Extraction of Mycobacterium tuberculosis DNA: a question of containment.Journal of clinical microbiology · 2005Article
- Viability testing of material derived from Mycobacterium tuberculosis prior to removal from a containment level-III laboratory as part of a Laboratory Risk Assessment Program.BMC infectious diseases · 2005Article
- Temporal analysis of archived samples indicates marked genetic changes in declining North Sea cod (Gadus morhua).Proceedings. Biological sciences · 2003Article
- Detection of a point mutation associated with high-level isoniazid resistance in Mycobacterium tuberculosis by using real-time PCR technology with 3'-minor groove binder-DNA probes.Journal of clinical microbiology · 2003Article
- Use of DNA extracts from Ziehl-Neelsen-stained slides for molecular detection of rifampin resistance and spoligotyping of Mycobacterium tuberculosis.Journal of clinical microbiology · 2003Article
- The efficacy of the heat killing of Mycobacterium tuberculosis.Journal of clinical pathology · 2002Article
- Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphism.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two criteria must be met before mycobacterial specimens can be tested by DNA amplification methods: (i) the sample must be rendered noninfectious, and (ii) the organisms must be lysed to free the DNA. Previous publications reporting DNA amplification of mycobacteria have concentrated on lysis and amplification procedures and have not addressed the issue of sample safety. We have shown that heating of samples below 100 degrees C may not consistently kill mycobacteria; however, heating at 100 degrees C in a boiling-water bath or a forced-air oven for a minimum of 5 min kills mycobacteria, including Mycobacterium thermoresistibile. Furthermore, heating at 100 degrees C for 30 min consistently lyses mycobacteria to produce short fragments of DNA that are suitable for amplification by PCR and strand displacement amplification. This procedure works with clinical samples digested by the n-acetyl cysteine-NaOH method as well as with suspensions of organisms in phosphate buffer. This paper also demonstrates the feasibility of using strand displacement amplification with clinical specimens.
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