Evidence map›Paper›PMID 7814492›Full record

ArticleJournal of clinical microbiology1994

Monitoring spread of Malassezia infections in a neonatal intensive care unit by PCR-mediated genetic typing.

A van Belkum, T Boekhout, R Bosboom

Open access · bronzeAbstract read
In one paragraph

Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 106 citations in OpenAlex.

  1. Journal of fungi (Basel, Switzerland) · 2022
    Review
  2. Susceptibilities ofHeliyon · 2020
    Article
  3. Frontiers in cellular and infection microbiology · 2020
    Review
  4. Article
  5. The Malassezia genus in skin and systemic diseases.Clinical microbiology reviews · 2012
    Review
  6. Article
  7. Malassezia pachydermatis carriage in dog owners.Emerging infectious diseases · 2005
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Molecular differentiation of seven Malassezia species.Journal of clinical microbiology · 2000
    Article
  15. Article
  16. Isolation of Malassezia furfur from a cat.Journal of clinical microbiology · 1999
    Article
  17. Epidemiology of nosocomial fungal infections.Clinical microbiology reviews · 1996
    Article
  18. Malassezia-Can it be Ignored?Indian journal of dermatology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

A van BelkumDepartment of Molecular Biology, Diagnostic Center SSDZ, Delft, The Netherlands.
T Boekhout
R Bosboom
DDL Diagnostic Laboratory · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Malassezia furfur and Malassezia pachydermatis were isolated from newborn children and incubators in a neonatal intensive care unit. To assess whether persistence or frequent import of the organisms was the cause of the elevated incidence, genetic typing of the strains was performed by PCR-mediated DNA fingerprinting. By using PCR primers aimed at repeat consensus motifs, six different genotypes could be detected in a collection of six M. furfur reference strains. In the case of 10 M. pachydermatis reference strains, nine different genotypes were detected by three different PCR assays. None of these assays could document genetic differences among the clinical isolates of either M. furfur or M. pachydermatis. On the basis of these results it is concluded that within the neonatal intensive care unit the longitudinal persistence of both an M. furfur and an M. pachydermatis strain has occurred and that Malassezia species can persist on incubator surfaces for prolonged periods of time. It can be concluded that PCR fingerprinting is a Malassezia typing procedure that is to be preferred over the analysis of chromosomal polymorphisms by pulsed-field gel electrophoresis in this genus.

Indexed as

Polymerase Chain ReactionBase SequenceBasidiomycotaCross InfectionDNA FingerprintingElectrophoresis, Gel, Pulsed-FieldGenotypeHumansInfant, NewbornIntensive Care Units, NeonatalMolecular Sequence Data

Identifiers

PMID7814492
PMCPMC264096
OpenAlexW1645491941

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.